Hormonal Stimulation of Phosphorylase Activity in the Rat Uterusin Vitro
| Autor: | Marjorie Crandall, Samuel L. Leonard |
|---|---|
| Rok vydání: | 1963 |
| Předmět: |
Serotonin
medicine.medical_specialty Epinephrine Phosphorylases Uterus Stimulation In Vitro Techniques Oxytocin chemistry.chemical_compound Glycogen phosphorylase Endocrinology Internal medicine medicine Humans Estradiol Glycogen urogenital system Muscles Research Phosphotransferases Muscle Smooth Hormones Rats Glucose medicine.anatomical_structure chemistry Female hormones hormone substitutes and hormone antagonists Histamine Hormone medicine.drug |
| Zdroj: | Endocrinology. 73:807-815 |
| ISSN: | 1945-7170 0013-7227 |
| DOI: | 10.1210/endo-73-6-807 |
| Popis: | Isolated uteri from estrogenprimed and spayed rats were allowed to contract isotonically for 45 min in a Krebs- Ringer-bicarbonate-glucose medium gassed with 95% O2:5% CO2. Hormones were then added to the bath and the uteri were assayed for active phosphorylase a and total phosphorylase t. Epinephrine increased phosphorylase a activity 16 % in uteri from spayed rats, 45 % in uteri estrogen-primed for 6 hr and 98% in uteri estrogen-primed for 48 hr. The increase was maximal within 2 min and the activity declined within 10 min. Chronic addition of epinephrine to the bath maintained significantly high levels of phosphorylase a for 10 min. Histamine, 5-hydroxytryptamine and oxytocin also increased uterine phosphorylase a within 2 min. The latter 2 substances produced sustained uterine contractions, whereas histamine and epinephrine caused relaxation. Uteri equilibrated in the medium without glucose had higher phosphorylase a levels than those equilibrated in the standard medium. Epinephrine added to glucose-f... |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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