Simple inoculum standardizing system for antimicrobial disk susceptibility tests

Autor:	E H Gerlach, M B Coyle, R W Hawkinson, Clyde Thornsberry, Daniel Amsterdam, Arthur L. Barry
Rok vydání:	1979
Předmět:	Microbiology (medical) Alternative methods Veterinary medicine Intralaboratory Staphylococcus Streptococcus Microbial Sensitivity Tests Biology Antimicrobial Anti-Bacterial Agents Microbiology Enterobacteriaceae Evaluation Studies as Topic Pseudomonas aeruginosa Research Article
Zdroj:	Journal of Clinical Microbiology. 10:910-918
ISSN:	1098-660X 0095-1137
DOI:	10.1128/jcm.10.6.910-918.1979
Popis:	A collaborative study was undertaken to evaluate a simple, convenient device which expedites inoculum standardization for antimicrobial disk susceptibility tests. The Inocupac system (Minnesota Mining & Manufacturing [3M] Co., St. Paul, Minn.) was used to perform disk tests in parallel with the standard Bauer-Kirby method. Five investigators tested 100 selected isolates, each in triplicate. Inter- and intralaboratory precision of both disk procedures was essentially comparable. The Inocupac system failed to consistently provide satisfactory growth with some streptococci, but when testing common gram-negative bacilli, staphylococci, and most enterococci, the Inocupac system gave zones about the same size (+/-2 mm) as the Bauer-Kirby procedure. Interpretive agreement between the two test systems varied from 90 to 99% with different antimicrobial agents, and repeated tests with the Bauer-Kirby method demonstrated the same degree of interpretive agreement. The Inocupac system is a valid alternative method for inoculating disk susceptibility tests.
Databáze:	OpenAIRE
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