Genome assembly and geospatial phylogenomics of the bed bug Cimex lectularius
| Autor: | Michael C. Schatz, Louis N. Sorkin, Mark E. Siddall, Michael Tessler, Alex Kentsis, Richard Baker, Saki Chan, Christopher E. Mason, Rob DeSalle, Paul J. Planet, Sabrina Simon, George Amato, Kevin Shianna, Mercer R. Brugler, Jeffrey A. Rosenfeld, Sergios-Orestis Kolokotronis, Vladyslav Kholodovych, Apurva Narechania, Ebrahim Afshinnekoo, Han Cao, Jorge Gandara, Russell E. Durrett, Alex Hastie, Michael Saghbini, Darryl Reeves, Ernest T. Lam, Jonathan Foox |
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| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
Male
Models Molecular 0301 basic medicine Bedbugs Science General Physics and Astronomy Sequence assembly Genome Article General Biochemistry Genetics and Molecular Biology Eating 03 medical and health sciences Bed bug Phylogenomics medicine Animals Humans Life Science Phylogeny Genetics Life Cycle Stages Multidisciplinary biology Sequence Analysis RNA Gene Expression Profiling fungi Chromosome Mapping Gene Expression Regulation Developmental General Chemistry biology.organism_classification medicine.disease Biosystematiek Blood 030104 developmental biology Biosystematics Female Wolbachia EPS Adaptation Cimex lectularius |
| Zdroj: | Nature Communications, Vol 7, Iss 1, Pp 1-10 (2016) Nature Communications 7 (2016) Nature Communications Nature Communications, 7 |
| ISSN: | 2041-1723 |
| Popis: | The common bed bug (Cimex lectularius) has been a persistent pest of humans for thousands of years, yet the genetic basis of the bed bug's basic biology and adaptation to dense human environments is largely unknown. Here we report the assembly, annotation and phylogenetic mapping of the 697.9-Mb Cimex lectularius genome, with an N50 of 971 kb, using both long and short read technologies. A RNA-seq time course across all five developmental stages and male and female adults generated 36,985 coding and noncoding gene models. The most pronounced change in gene expression during the life cycle occurs after feeding on human blood and included genes from the Wolbachia endosymbiont, which shows a simultaneous and coordinated host/commensal response to haematophagous activity. These data provide a rich genetic resource for mapping activity and density of C. lectularius across human hosts and cities, which can help track, manage and control bed bug infestations. The common bedbug is a pest for humans, yet its molecular biology is poorly understood. Here, the authors sequence the common bedbug genome and profile gene expression across all life stages to show major changes in gene expression after feeding on human blood. |
| Databáze: | OpenAIRE |
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