Identification of a new class of protein kinases represented by eukaryotic elongation factor-2 kinase
| Autor: | Toni Gestone Parmer, F. Joseph Germino, Maxim V. Dorovkov, Hediye Erdjument-Bromage, Charmaine E. Mendola, Paul Tempst, Michael D. Ward, Karen S. Pavur, William N. Hait, C. Robert Prostko, Martin Wiedmann, Alexey G. Ryazanov |
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| Rok vydání: | 1997 |
| Předmět: |
Elongation Factor 2 Kinase
Reticulocytes Transcription Genetic Molecular Sequence Data Protozoan Proteins Biology Polymerase Chain Reaction SH3 domain MAP2K7 Mice Adenosine Triphosphate Animals Humans Dictyostelium Amino Acid Sequence c-Raf Cloning Molecular Caenorhabditis elegans education Conserved Sequence DNA Primers education.field_of_study Binding Sites Multidisciplinary Base Sequence Sequence Homology Amino Acid Cyclin-dependent kinase 2 Biological Sciences Recombinant Proteins Rats Biochemistry Protein Biosynthesis Calcium-Calmodulin-Dependent Protein Kinases biology.protein Cyclin-dependent kinase complex Rabbits Elongation Factor-2 Kinase Cyclin-dependent kinase 7 Casein kinase 2 |
| Zdroj: | Proceedings of the National Academy of Sciences. 94:4884-4889 |
| ISSN: | 1091-6490 0027-8424 |
| DOI: | 10.1073/pnas.94.10.4884 |
| Popis: | The several hundred members of the eukaryotic protein kinase superfamily characterized to date share a similar catalytic domain structure, consisting of 12 conserved subdomains. Here we report the existence and wide occurrence in eukaryotes of a protein kinase with a completely different structure. We cloned and sequenced the human, mouse, rat, and Caenorhabditis elegans eukaryotic elongation factor-2 kinase (eEF-2 kinase) and found that with the exception of the ATP-binding site, they do not contain any sequence motifs characteristic of the eukaryotic protein kinase superfamily. Comparison of different eEF-2 kinase sequences reveals a highly conserved region of ≈200 amino acids which was found to be homologous to the catalytic domain of the recently described myosin heavy chain kinase A (MHCK A) from Dictyostelium. This suggests that eEF-2 kinase and MHCK A are members of a new class of protein kinases with a novel catalytic domain structure. |
| Databáze: | OpenAIRE |
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