Characterization of Yersinia enterocolitica strains potentially virulent for humans and animals in river water
| Autor: | Agata Bancerz-Kisiel, Elżbieta Terech-Majewska, J. Pajdak, A. Szczerba-Turek, Karolina Grabowska, Aleksandra Platt-Samoraj |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Serotype Yersinia Infections Virulence Factors 030106 microbiology Virulence Sewage Applied Microbiology and Biotechnology Microbiology 03 medical and health sciences Bacterial Proteins Rivers Multiplex polymerase chain reaction Animals Humans media_common.cataloged_instance European union Yersinia enterocolitica Pathogen media_common biology business.industry General Medicine biology.organism_classification France business Bacteria Biotechnology |
| Zdroj: | Journal of Applied Microbiology. 121:554-560 |
| ISSN: | 1364-5072 |
| Popis: | AIMS The aim of this study was to isolate and identify potentially pathogenic strains of Yersinia enterocolitica in water samples collected from the upstream section of the Drweca River in Poland. METHODS AND RESULTS Thirty-nine water samples were collected. Strains were isolated, identified with the use of the API(®) 20E test kit (Biomerieux, Marcy l'Etoile, France) at 37°C, serotyped and subjected to a molecular analysis. Multiplex PCR was carried out to amplify three virulence genes: ail, ystA and ystB. Fragments of ail and ystA genes were not identified in the genetic material of the analysed strains. The ystB gene was identified in four strains. Yersinia enterocolitica strains of biotype 1A, which contain the ystB gene, may cause gastrointestinal problems. CONCLUSIONS In our study, Y. enterocolitica strains of biotype 1A/ystB with serotypes 0 : 3, 0 : 5 and 0 : 8 were identified in samples collected from the Drweca River which flows through the areas protected by Natura 2000, one of the largest networks of nature conservation areas in the European Union. The presence of Y. enterocolitica in the Drweca River indicates that the analysed bacteria colonize natural water bodies. SIGNIFICANCE AND IMPACT OF THE STUDY Most research focuses on food or sewage as a source of Y. enterocolitica infections. Little is known about the occurrence of this pathogen in natural waters. Our results show that natural waters are also a potential threat to human and animal health. |
| Databáze: | OpenAIRE |
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