Analysis of the expression of human bitter taste receptors in extraoral tissues
Autor: | Kangmin Duan, Rajinder P. Bhullar, Shyamala Dakshinamurti, Anurag Singh Sikarwar, Nisha Singh, Appalaraju Jaggupilli, Jasbir D. Upadhyaya, Makoto Arakawa, Prashen Chelikani |
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Rok vydání: | 2016 |
Předmět: |
0301 basic medicine
Pathology medicine.medical_specialty Clinical Biochemistry Stimulation Bronchi Respiratory Mucosa Biology Calcium in biology Flow cytometry Receptors G-Protein-Coupled 03 medical and health sciences Gene expression medicine Humans Calcium Signaling Receptor Molecular Biology Gene medicine.diagnostic_test Muscle Smooth Cell Biology General Medicine 3. Good health Cell biology 030104 developmental biology Gene Expression Regulation Organ Specificity Cancer cell TAS2R14 |
Zdroj: | Molecular and cellular biochemistry. 426(1-2) |
ISSN: | 1573-4919 |
Popis: | The 25 bitter taste receptors (T2Rs) in humans perform a chemosensory function. However, very little is known about the level of expression of these receptors in different tissues. In this study, using nCounter gene expression we analyzed the expression patterns of human TAS2R transcripts in cystic fibrosis bronchial epithelial (CuFi-1), normal bronchial epithelial (NuLi-1), airway smooth muscle (ASM), pulmonary artery smooth muscle (PASM), mammary epithelial, and breast cancer cells. Our results suggest a specific pattern of TAS2R expression with TAS2R3, 4, 5, 10, 13, 19, and 50 transcripts expressed at moderate levels and TAS2R14 and TAS2R20 (or TASR49) at high levels in the various tissues analyzed. This pattern of expression is mostly independent of tissue origin and the pathological state, except in cancer cells. To elucidate the expression at the protein level, we pursued flow cytometry analysis of select T2Rs from CuFi-1 and NuLi-1 cells. The expression levels observed at the gene level by nCounter analysis correlate with the protein levels for the T2Rs analyzed. Next, to assess the functionality of the expressed T2Rs in these cells, we pursued functional assays measuring intracellular calcium mobilization after stimulation with the bitter compound quinine. Using PLC inhibitor, U-73122, we show that the calcium mobilized in these cells predominantly takes place through the Quinine-T2R-Gαβγ-PLC pathway. This report will accelerate studies aimed at analyzing the pathophysiological function of T2Rs in different extraoral tissues. |
Databáze: | OpenAIRE |
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