Antitumor Effect of Sclerostin against Osteosarcoma
| Autor: | Takeshi Uemura, Hiroyuki Kato, Kazushige Yoshida, Takayuki Kamanaka, Takashi Takizawa, Atsushi Tanaka, Hirokazu Ideta, Jun Sasaki, Munehisa Kito, Kaoru Aoki, Akira Takazawa, Shuichiro Suzuki, Hisao Haniu, Naoto Saito, Masanori Okamoto, Jun Takahashi, Atsushi Sobajima, Yasuo Yoshimura |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
musculoskeletal diseases
Cancer Research Cell Wnt pathway sclerostin Article chemistry.chemical_compound osteosarcoma medicine Extracellular Doxorubicin neoplasms RC254-282 Wnt signaling pathway Neoplasms. Tumors. Oncology. Including cancer and carcinogens medicine.disease Transplantation medicine.anatomical_structure Oncology chemistry Cell culture Cancer research Osteosarcoma Sclerostin medicine.drug |
| Zdroj: | Cancers, Vol 13, Iss 6015, p 6015 (2021) Cancers; Volume 13; Issue 23; Pages: 6015 Cancers |
| ISSN: | 2072-6694 |
| Popis: | Various risk factors and causative genes of osteosarcoma have been reported in the literature; however, its etiology remains largely unknown. Bone formation is a shared phenomenon in all types of osteosarcomas, and sclerostin is an extracellular soluble factor secreted by osteocytes that prevents bone formation by inhibiting the Wnt signaling pathway. We aimed to investigate the antitumor effect of sclerostin against osteosarcoma. Osteosarcoma model mice were prepared by transplantation into the dorsal region of C3H/He and BALB/c-nu/nu mice using osteosarcoma cell lines LM8 (murine) and 143B (human), respectively. Cell proliferations were evaluated by using alamarBlue and scratch assays. The migratory ability of the cells was evaluated using a migration assay. Sclerostin was injected intraperitoneally for 7 days to examine the suppression of tumor size and extension of survival. The administration of sclerostin to osteosarcoma cells significantly inhibited the growth and migratory ability of osteosarcoma cells. Kaplan–Meier curves and survival data demonstrated that sclerostin significantly inhibited tumor growth and improved survival. Sclerostin suppressed the proliferative capacity and migratory ability of osteosarcoma cells. Osteosarcoma model mice inhibited tumor growth and prolonged survival periods by the administration of sclerostin. The effect of existing anticancer drugs such as doxorubicin should be investigated for future clinical applications. Article Cancers 13(23) : 6015(2021) |
| Databáze: | OpenAIRE |
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