Does an increased body mass index affect endometrial gene expression patterns in infertile patients? A functional genomics analysis

Autor: Patricia Diaz-Gimeno, José Bellver, Diana Valbuena, Patricia Sebastian-Leon, Maria Ruiz-Alonso, Carlos Simón, Sergio Cabanillas, Ruth B. Lathi, M. Shah, Amy Schutt, C.T. Valdes, Ioanna A. Comstock
Rok vydání: 2017
Předmět:
Genetic Markers
0301 basic medicine
Oncology
Infertility
medicine.medical_specialty
Endometrium
California
Body Mass Index
Miscarriage
03 medical and health sciences
0302 clinical medicine
Pregnancy
Risk Factors
Internal medicine
medicine
Humans
Gene Regulatory Networks
Genetic Predisposition to Disease
Embryo Implantation
Obesity
Prospective Studies
Prospective cohort study
Gynecology
030219 obstetrics & reproductive medicine
business.industry
Gene Expression Profiling
Female infertility
Case-control study
Obstetrics and Gynecology
Genomics
medicine.disease
Texas
Abortion
Spontaneous
Fertility
Phenotype
030104 developmental biology
medicine.anatomical_structure
Gene Expression Regulation
Reproductive Medicine
Spain
Case-Control Studies
Female
Transcriptome
business
Infertility
Female
Body mass index
Zdroj: Fertility and Sterility. 107:740-748.e2
ISSN: 0015-0282
0220-5866
DOI: 10.1016/j.fertnstert.2016.11.009
Popis: Objective To analyze the transcriptomic profile of endometrial gene alterations during the window of implantation in infertile obese patients. Design Multicenter, prospective, case–control study. Setting Three academic medical centers for reproductive medicine. Patient(s) Infertile patients, stratified into body mass index (BMI) categories according to the World Health Organization guidelines, were included in the study. Intervention(s) Endometrial samples were obtained from women undergoing standardized estrogen and P replacement cycles after 5 days of vaginal P supplementation. Main Outcome Measure(s) To identify endometrial gene expression alterations that occur during the window of implantation in infertile obese patients as compared with infertile normal-weight controls using a microarray analysis. Result(s) XCL1 , XCL2 , HMHA1 , S100A1 , KLRC1 , COTL1 , COL16A1 , KRT7 , and MFAP5 are significantly dysregulated during the window of implantation in the receptive endometrium of obese patients. COL16A1 , COTL1 , HMHA1 , KRCL1 , XCL1 , and XCL2 were down-regulated and KRT7 , MFAP5 , and S100A1 were up-regulated in the endometrium of obese patients. These genes are mainly involved in chemokine, cytokine, and immune system activity and in the structural extracellular matrix and protein-binding molecular functions. Conclusion(s) Obesity is associated with significant endometrial transcriptomic differences as compared with non-obese subjects. Altered endometrial gene expression in obese patients may contribute to the lower implantation rates and increased miscarriage rates seen in obese infertile patients. Clinical Trial Registration Number NCT02205866.
Databáze: OpenAIRE
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