Selection of Trypanosoma cruzi clonal genotypes (Clonet 20 and 39) isolated from Bolivian triatomines following subculture in liquid medium
| Autor: | Nina Yacsik, Marie-France Bosseno, Fernando Vargas, Simone Frédérique Brenière |
|---|---|
| Přispěvatelé: | Institut de Recherche pour le Développement - Bolivie (IRD-Bolivie), LARDEUX, Frédéric |
| Jazyk: | angličtina |
| Rok vydání: | 2000 |
| Předmět: |
SELECTION
CULTURE IN VITRO clonal genotypes ISOENZYME lcsh:QR1-502 [SDV.BBM.BM] Life Sciences [q-bio]/Biochemistry Molecular Biology/Molecular biology Polymerase Chain Reaction lcsh:Microbiology law.invention Feces 0302 clinical medicine law Genotype TECHNIQUE PCR Parasite hosting Triatoma Polymerase chain reaction Triatoma infestans 0303 health sciences 3. Good health GENOTYPE HYBRIDATION Rhodnius MALADIE DE CHAGAS Triatominae ANALYSE GENETIQUE [SDV.MP.PAR] Life Sciences [q-bio]/Microbiology and Parasitology/Parasitology Microbiology (medical) Chagas disease Bolivia lcsh:Arctic medicine. Tropical medicine lcsh:RC955-962 Trypanosoma cruzi 030231 tropical medicine Biology 03 medical and health sciences parasitic diseases medicine ETUDE COMPARATIVE Animals INSECTE NUISIBLE [SDV.MP.PAR]Life Sciences [q-bio]/Microbiology and Parasitology/Parasitology PARASITE 030304 developmental biology VECTEUR [SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry Molecular Biology/Molecular biology medicine.disease biology.organism_classification Virology culture Culture Media Insect Vectors [SDV.BA.ZI]Life Sciences [q-bio]/Animal biology/Invertebrate Zoology BOLIVIE [SDV.BA.ZI] Life Sciences [q-bio]/Animal biology/Invertebrate Zoology Hybridization Genetic ETUDE EXPERIMENTALE Subculture (biology) |
| Zdroj: | Memórias do Instituto Oswaldo Cruz Memórias do Instituto Oswaldo Cruz, Instituto Oswaldo Cruz, Ministério da Saúde, 2000, 95 (5), pp.601-607 Memórias do Instituto Oswaldo Cruz, Volume: 95, Issue: 5, Pages: 601-607, Published: OCT 2000 Memórias do Instituto Oswaldo Cruz., Vol 95, Iss 5, Pp 601-607 (2000) Europe PubMed Central |
| ISSN: | 0074-0276 1678-8060 |
| Popis: | International audience; Previous studies showed that two groups of Trypanosoma cruzi clonal genotypes named clonet 20 and clonet 39 were predominant in Triatoma infestans, the unique vector of Chagas disease in Bolivia. These groups of clones correspond to distinct genetic clusters. These clonets were detected in T. infestans and Rhodnius pictipes fecal samples before isolation and after culture by kDNA PCR (polymerase chain reaction) and hybridization of the amplified products with clonet specific kDNA probes named 20 and 39 as previously reported. Forty eight T. infestans and three R. pictipes infected insects captured at random in different Bolivian departments were proceeded. As previously reported the direct identification of the two major clonet in fecal samples allowed the detection of abundant mixed infections : 41% in the original sample, however after culture, only 6% of mixed infections were initially detected. Among the 21 parasite stocks isolated from digestive tracts where mixed infections were initially detected (clonet 20 + 39) clonet 20 alone was detected in 81% of them. This result clearly showed that the culture step selected clonet 20 parasites over those belonging to clonet 39. The taxonomic status of the isolated stocks was also confirmed by isoenzyme typing, and correlation was observed between clustering topology and hybridization patterns with the probes 20 and 39. (Résumé d'auteur) |
| Databáze: | OpenAIRE |
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