Ethyl acetate extract of Hypericum japonicum induces apoptosis via the mitochondria-dependent pathway in vivo and in vitro
| Autor: | Youqin Chen, Qunchuan Zhuang, Jun Peng, Xuzheng Chen, Jing Li, Jiumao Lin, Faze Lai, Xindeng Lin |
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| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
Cancer Research
Cell Caspase 3 Apoptosis Mitochondrion Acetates Biochemistry liver cancer Mice Bcl-2-associated X protein In vivo Cell Line Tumor Genetics medicine Animals Anticarcinogenic Agents Humans Molecular Biology HepG2 cells bcl-2-Associated X Protein Hypericum japonicum Membrane Potential Mitochondrial biology Plant Extracts Body Weight Liver Neoplasms Articles Hep G2 Cells biology.organism_classification Molecular biology Xenograft Model Antitumor Assays Caspase 9 Mitochondria medicine.anatomical_structure Oncology Gene Expression Regulation herbal medicine biology.protein Molecular Medicine Signal transduction Hypericum Signal Transduction |
| Zdroj: | Molecular Medicine Reports |
| ISSN: | 1791-3004 1791-2997 |
| Popis: | The widely-used Chinese medicinal herb Hypericum japonicum, also known as Hypericum japonicum Thunb or Tianjihuang, displays potent anti‑carcinogenic effects against liver cancer. However, the molecular mechanism underlying the therapeutic effects of Hypericum japonicum remains to be elucidated. The present study investigated the in vivo efficacy of ethyl acetate extract of Hypericum japonicum (EAEHJ) against tumor growth in an H22 cell‑bearing liver cancer mouse model. Treatment with EAEHJ significantly reduced tumor weight, but had no effect on murine body weight. The results of the present study also showed that EAEHJ induced H22 cell apoptosis in vivo. In addition, the anti‑carcinogenic effects of EAEHJ were investigated in vitro. The results of the present study demonstrate that both phospholipid asymmetry in the plasma membrane and mitochondrial membrane potential were deregulated in HepG2 human hepatoma cells, following treatment with EAEHJ. Treatment with EAEHJ also increased the ratio of pro‑apoptotic B‑cell lymphoma 2 (Bcl‑2)‑associated X protein (Bax) to anti‑apoptotic Bcl‑2, and activated the caspase‑9 signaling pathway. These results suggest that EAEHJ is able to trigger the apoptosis of liver cancer cells via the mitochondria-dependent pathway. |
| Databáze: | OpenAIRE |
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