Effects of standardized isopropanolic black cohosh and estrogen on salivary function in ovariectomized rats
| Autor: | Jing Jia, Yunmeng Da, Hai Jiang, Ke Wang, Lihua Qin, Jing Zhang, Wenpei Bai, Yao Liu, Shuya Liu, Yi Zhang, Kaiyu Niu, Wenjuan Wang |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
medicine.medical_specialty Cimicifuga medicine.drug_class Ovariectomy Black cohosh Saliva secretion Xerostomia 2-Propanol Rats Sprague-Dawley 03 medical and health sciences 0302 clinical medicine Actaea racemosa stomatognathic system Internal medicine Muscarinic acetylcholine receptor medicine Animals Saliva Pharmacology Receptor Muscarinic M2 biology business.industry Plant Extracts Receptor Muscarinic M1 Estrogens 030206 dentistry General Medicine medicine.disease biology.organism_classification Submandibular gland Rats Menopause stomatognathic diseases Disease Models Animal 030104 developmental biology Endocrinology medicine.anatomical_structure Estrogen Ovariectomized rat Solvents Female business |
| Zdroj: | Biomedicinepharmacotherapy = Biomedecinepharmacotherapie. 97 |
| ISSN: | 1950-6007 |
| Popis: | Oral dryness is a common feature in menopausal women. Estrogen therapy can relieve this symptom; however, the underlying mechanism was not clear. Standardized isopropanolic black cohosh (Actaea racemosa; Remifemin) can also relieve menopausal symptoms, such as hot flashes and sweating. Our previous study showed that standardized isopropanolic black cohosh could protect the submandibular gland structure. To investigate the effects and possible mechanisms of action of estrogen and standardized isopropanolic black cohosh on submandibular gland function in ovariectomized (OVX) rats, we measured body weight, daily water consumption, and blood flow in the submandibular glands. Immunohistochemistry and western blotting were used to detect the expression of muscarinic acetylcholine receptors 1 (M1) and 3 (M3), and aquaporin 5 (AQP5) in the submandibular gland. OVX increased daily water consumption and reduced vasodilation in the submandibular gland. It suggested that ovariectomy could damage the salviary function. Moreover, the expression of M1 and M3 receptors decreased, whereas that of AQP5 increased. These changes may explain the dysfunction of saliva secretion in menopause. Estrogen and standardized isopropanolic black cohosh treatment had the same effect on daily water consumption and vasodilation in the submandibular gland. It indicated that estrogen and standardized isopropanolic black cohosh could relieve oral dryness in menopause. However, the mechanism of the two treatments may differ because standardized isopropanolic black cohosh only protected against changes in M1 expression, whereas estrogen protected against variations in M1, M3, and AQP5 expression. |
| Databáze: | OpenAIRE |
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