Whole-genome sequencing of the efficient industrial fuel-ethanol fermentative Saccharomyces cerevisiae strain CAT-1
| Autor: | Robert W. Shafer, Mostafa Ronaghi, Antonio Joaquim de Oliveira, Henrique Amorim, Chunlin Wang, Boris U. Stambuk, Roxana Jalili, Baback Gharizadeh, Avi Robinson-Mosher, Pål Nyrén, Ronald W. Davis, Sarah E. Pierce, Farbod Babrzadeh, Luiz Carlos Basso, Shadi Shokralla |
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| Rok vydání: | 2011 |
| Předmět: |
Transposable element
Saccharomyces cerevisiae Gene Dosage Genome Saccharomyces Polymorphism Single Nucleotide DNA sequencing Loss of heterozygosity Species Specificity Genetics Molecular Biology Gene Phylogeny Whole genome sequencing biology Ethanol General Medicine Sequence Analysis DNA biology.organism_classification Diploidy Biofuels Fermentation Genome Fungal Biotechnology |
| Zdroj: | Molecular genetics and genomics : MGG. 287(6) |
| ISSN: | 1617-4623 |
| Popis: | The Saccharomyces cerevisiae strains widely used for industrial fuel-ethanol production have been developed by selection, but their underlying beneficial genetic polymorphisms remain unknown. Here, we report the draft whole-genome sequence of the S. cerevisiae strain CAT-1, which is a dominant fuel-ethanol fermentative strain from the sugarcane industry in Brazil. Our results indicate that strain CAT-1 is a highly heterozygous diploid yeast strain, and the ~12-Mb genome of CAT-1, when compared with the reference S228c genome, contains ~36,000 homozygous and ~30,000 heterozygous single nucleotide polymorphisms, exhibiting an uneven distribution among chromosomes due to large genomic regions of loss of heterozygosity (LOH). In total, 58 % of the 6,652 predicted protein-coding genes of the CAT-1 genome constitute different alleles when compared with the genes present in the reference S288c genome. The CAT-1 genome contains a reduced number of transposable elements, as well as several gene deletions and duplications, especially at telomeric regions, some correlated with several of the physiological characteristics of this industrial fuel-ethanol strain. Phylogenetic analyses revealed that some genes were likely associated with traits important for bioethanol production. Identifying and characterizing the allelic variations controlling traits relevant to industrial fermentation should provide the basis for a forward genetics approach for developing better fermenting yeast strains. |
| Databáze: | OpenAIRE |
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