Fast Collisional Lipid Transfer Among Polymer-Bounded Nanodiscs
| Autor: | Cécile Breyton, Anne L. Martel, Rodrigo Cuevas Arenas, Bartholomäus Danielczak, Lionel Porcar, Christine Ebel, Sandro Keller |
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| Přispěvatelé: | Molecular Biophysics, University of Kaiserslautern [Kaiserslautern], Institut Laue-Langevin (ILL), Institut de biologie structurale (IBS - UMR 5075 ), Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019])-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA), ILL, Université Grenoble Alpes [2016-2019] (UGA [2016-2019])-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Centre National de la Recherche Scientifique (CNRS) |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Multidisciplinary [SDV.BBM.BS]Life Sciences [q-bio]/Biochemistry Molecular Biology/Structural Biology [q-bio.BM] Chemistry Vesicle Bilayer Membrane lipids Kinetics Phospholipid 7. Clean energy Article 03 medical and health sciences chemistry.chemical_compound 030104 developmental biology Membrane Förster resonance energy transfer Biochemistry Chemical physics lipids (amino acids peptides and proteins) Dynamic equilibrium |
| Zdroj: | Scientific Reports Scientific Reports, 2017, 7, pp.45875. ⟨10.1038/srep45875⟩ 'Scientific Reports ', vol: 7, pages: 45875-1-45875-8 (2017) Scientific Reports, Nature Publishing Group, 2017, 7, pp.45875. ⟨10.1038/srep45875⟩ |
| ISSN: | 2045-2322 |
| DOI: | 10.1038/srep45875 |
| Popis: | Some styrene/maleic acid (SMA) copolymers solubilise membrane lipids and proteins to form polymer-bounded nanodiscs termed SMA/lipid particles (SMALPs). Although SMALPs preserve a lipid-bilayer core, they appear to be more dynamic than other membrane mimics. We used time-resolved Förster resonance energy transfer and small-angle neutron scattering to determine the kinetics and the mechanisms of phospholipid transfer among SMALPs. In contrast with vesicles or protein-bounded nanodiscs, SMALPs exchange lipids not only by monomer diffusion but also by fast collisional transfer. Under typical experimental conditions, lipid exchange occurs within seconds in the case of SMALPs but takes minutes to days in the other bilayer particles. The diffusional and second-order collisional exchange rate constants for SMALPs at 30 °C are kdif = 0.287 s−1 and kcol = 222 M−1s−1, respectively. Together with the fast kinetics, the observed invariability of the rate constants with probe hydrophobicity and the moderate activation enthalpy of ~70 kJ mol−1 imply that lipids exchange through a “hydrocarbon continuum” enabled by the flexible nature of the SMA belt surrounding the lipid-bilayer core. Owing to their fast lipid-exchange kinetics, SMALPs represent highly dynamic equilibrium rather than kinetically trapped membrane mimics, which has important implications for studying protein/lipid interactions in polymer-bounded nanodiscs. |
| Databáze: | OpenAIRE |
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