Fluorescence of Candida in diagnosis of oral candidiasis
| Autor: | LR Kumaraswamy Naik, Lal P Madathil, Pushparaja Shetty, Vimal Kumar Karnaker, Sarosh E Shroff, M S Krishna Prasad |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Pathology medicine.medical_specialty H&E stain Papanicolaou stain Periodic acid–Schiff stain Biology periodic acid Schiff Stain Microbiology Agar plate 03 medical and health sciences 0302 clinical medicine Cytology medicine General Dentistry Candida 030102 biochemistry & molecular biology General Medicine Fluorescence lcsh:RK1-715 Autofluorescence lcsh:Dentistry 030220 oncology & carcinogenesis Papanicolaou cytology |
| Zdroj: | Indian Journal of Dental Research, Vol 27, Iss 6, Pp 618-622 (2016) |
| ISSN: | 0970-9290 |
| DOI: | 10.4103/0970-9290.199592 |
| Popis: | Background: Many pathogenic fungi fluoresce in hematoxylin and eosin stained sections, and Papanicolaou (PAP)-stained smears under ultraviolet illumination. In theory, this phenomenon could aid in the diagnosis of common fungal infections without the delay which is usually associated with special stains. Objective: To evaluate the role of fluorescence as a rapid screening technique for oral infections caused by Candida organisms in exfoliative smears of oral candidiasis. Materials and Methods: Two smears and one swab were collected from each of 62 clinically diagnosed cases of oral candidiasis. Smears were stained with (PAP) and periodic acid–Schiff stain (PAS). Both smears were evaluated under light microscopy (LM). Later, PAP smears were observed under fluorescent microscopy (PAP-FM). The swab was inoculated on Sabouraud's agar plate. Each technique was evaluated for sensitivity and specificity. Results: It was found that the PAS-stained smears were more reliable for detection of Candida species than other methods (sensitivity = 100%; specificity = 66.7%). The PAP-LM and PAP-FM showed less sensitivity (67.9% and 85.7%) and specificity (66.7% and 33.3%), respectively. Combined results of both light and fluorescent microscopy of PAP (LM + FM) showed increased sensitivity (89.3%) but reduced specificity (16.7%). Conclusion: PAP autofluorescence is less sensitive than PAS, still it accentuates the distinct morphological features of Candida. |
| Databáze: | OpenAIRE |
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