TaWRKY74 participates copper tolerance through regulation of TaGST1 expression and GSH content in wheat
| Autor: | Tiancai Guo, Gezi Li, Jin Liu, Shi-Juan Chen, Yong-Xing Zheng, Yonghua Wang, Pengfei Wang, Guozhang Kang |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Health
Toxicology and Mutagenesis 0211 other engineering and technologies 02 engineering and technology 010501 environmental sciences glutathione S-transferase 1 gene 01 natural sciences Environmental pollution chemistry.chemical_compound Two-Hybrid System Techniques Yeasts Luciferase GE1-350 Hydrogen peroxide Gene Triticum aestivum L Triticum 0105 earth and related environmental sciences Glutathione Transferase Plant Proteins chemistry.chemical_classification 021110 strategic defence & security studies Public Health Environmental and Occupational Health General Medicine Glutathione Malondialdehyde Pollution Yeast Copper stress Environmental sciences Enzyme chemistry Biochemistry TD172-193.5 Shoot TaWRKY74 transcription factor Glutathione synthesis Copper Transcription Factors |
| Zdroj: | Ecotoxicology and Environmental Safety, Vol 221, Iss, Pp 112469-(2021) |
| ISSN: | 1090-2414 |
| Popis: | Glutathione S-transferase (GST) is the key enzyme in glutathione (GSH) synthesis, and plays a crucial role in copper (Cu) detoxification. Nonetheless, its regulatory mechanisms remain largely unclear. In this study, we identified a Cu-induced glutathione S-transferase 1 (TaGST1) gene in wheat. Yeast one-hybrid (Y1H) screened out TaWRKY74, which was one member from the WRKY transcription factor family. The bindings between TaGST1 promoter and TaWRKY74 were further verified by using another Y1H and luciferase assays. Expression of TaWRKY74 was induced more than 30-folds by Cu stress. Functions of TaWRKY74 were tested by using transiently silence methods. In transiently TaWRKY74-silenced wheat plants, TaWRKY74 and TaGST1 expression, GST activity, and GSH content was significantly inhibited by 25.68%, 19.88%, 27.66%, and 12.68% in shoots, and 53.81%, 52.11%, 23.47%, and 17.11% in roots, respectively. However, contents of hydrogen peroxide, malondialdehyde, or Cu were significantly increased by 2.58%, 12.45%, or 37.74% in shoots, and 25.24%, 53.84%, and 103.99% in roots, respectively. Notably, exogenous application of GSH reversed the adverse effects of transiently TaWRKY74-silenced wheat plants during Cu stress. Taken together, our results suggesting that TaWRKY74 regulated TaGST1 expression and affected GSH accumulation under Cu stress, and could be useful to ameliorate Cu toxicity for crop food safety. |
| Databáze: | OpenAIRE |
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