Effects of extraction methods on the physicochemical characteristics and biological activities of polysaccharides from okra (Abelmoschus esculentus)
| Autor: | Wen Liu, Qing Zhang, Xi-Rui Nie, Wen Qin, Shang Lin, Su Yan, Qiao-Hong Han, Qin Yuan, Ding-Tao Wu, Li Zhao, Yuan Fu, Derong Lin |
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| Rok vydání: | 2019 |
| Předmět: |
Antioxidant
medicine.medical_treatment 02 engineering and technology Polysaccharide Biochemistry 03 medical and health sciences Abelmoschus Polysaccharides Structural Biology medicine Monosaccharide Glycoside Hydrolase Inhibitors Food science Molecular Biology 030304 developmental biology chemistry.chemical_classification 0303 health sciences Molecular mass biology Extraction (chemistry) alpha-Glucosidases Water extraction General Medicine 021001 nanoscience & nanotechnology biology.organism_classification Hot water extraction chemistry alpha-Amylases 0210 nano-technology |
| Zdroj: | International Journal of Biological Macromolecules. 127:178-186 |
| ISSN: | 0141-8130 |
| DOI: | 10.1016/j.ijbiomac.2019.01.042 |
| Popis: | The impacts of three extraction techniques, including hot water extraction (HWE), pressurized water extraction (PWE), and microwave assisted extraction (MAE), on the physicochemical characteristics, antioxidant activities, in vitro binding properties, and in vitro inhibitory activities on α-amylase and α-glucosidase of okra polysaccharides (OPPs) were investigated and compared. The extraction yields, constituent monosaccharides, and FT-IR spectra of OPP-W, OPP-P, and OPP-M extracted by HWE, PWE, and MAE, respectively, were similar. However, their molecular weights, intrinsic viscosities, uronic acids, and degree of esterification were different. Furthermore, results showed that OPP-W, OPP-P, and OPP-M exhibited remarkable antioxidant activities, binding capacities, and inhibitory activities on α-amylase and α-glucosidase. Indeed, the antioxidant activities of OPP-W were significantly lower than those of OPP-M and OPP-P, which might be attributed to the low molecular weights and high contents of unmethylated galacturonic acid of OPP-P and OPP-M. However, the binding capacities and inhibitory activities on α-amylase and α-glucosidase of OPP-W and OPP-P were similar, but significantly higher than those of OPP-M, which might be attributed to the low molecular weights of OPP-M. Results suggested that the PWE method could be a good potential technique for the extraction of OPPs with high bioactivities for industrial applications. |
| Databáze: | OpenAIRE |
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