L. fermentum CECT 5716 prevents stress-induced intestinal barrier dysfunction in newborn rats

Autor: P.-A. Grohard, A. Ligneul, Philippe Hulin, Fabian Docagne, P. Paul-Gilloteaux, Antoine P. Fournier, Philippe Naveilhan, Philippe Aubert, P. Le Ruyet, Michel Neunlist, T Durand, Tiphaine Vanhaecke, Hélène Boudin, C. Fressange-Mazda
Přispěvatelé: Scientific Committee for Consumer Safety (SCCS) (SCCS), Université d'Evry Val d'Essonne Laboratoire d'études des milieux nanométriques, Université d'Évry-Val-d'Essonne (UEVE), Institut des Biomolécules Max Mousseron [Pôle Chimie Balard] (IBMM), Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)-Université de Montpellier (UM)-Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM), Structure fédérative de recherche François Bonamy (SFR François Bonamy), Université de Nantes (UN)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche en Santé de l'Université de Nantes (IRS-UN), Lactalis Nutrition Europe, Groupe Lactalis, UMR 643, Institut National de la Santé et de la Recherche Médicale (INSERM), U913, Laboratoire d'étude des milieux nanométriques (LEMN), Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM)-Institut de Chimie du CNRS (INC)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)
Jazyk: angličtina
Rok vydání: 2017
Předmět:
0301 basic medicine
Limosilactobacillus fermentum
Physiology
Lactobacillus fermentum
Colon
Gastrointestinal Diseases
Pharmacology
digestive system
Permeability
law.invention
Rats
Sprague-Dawley
03 medical and health sciences
Probiotic
Immune system
Western blot
law
medicine
Animals
Colitis
Intestinal Mucosa
ComputingMilieux_MISCELLANEOUS
Intestinal permeability
biology
medicine.diagnostic_test
Endocrine and Autonomic Systems
Maternal Deprivation
Probiotics
Gastroenterology
food and beverages
Epithelial Cells
medicine.disease
biology.organism_classification
Small intestine
3. Good health
030104 developmental biology
medicine.anatomical_structure
Animals
Newborn
Immunology
Exploratory Behavior
Zonula Occludens-1 Protein
Cytokine secretion
Female
[SDV.AEN]Life Sciences [q-bio]/Food and Nutrition
Stress
Psychological
Zdroj: Neurogastroenterology & Motility
Neurogastroenterology & Motility, Wiley, 2017, 29 (8), ⟨10.1111/nmo.13069⟩
Neurogastroenterology & Motility, 2017, 29 (8), ⟨10.1111/nmo.13069⟩
ISSN: 1350-1925
1365-2982
DOI: 10.1111/nmo.13069⟩
Popis: Background Intestinal epithelial barrier (IEB) dysfunction plays a critical role in various intestinal disorders affecting infants and children, including the development of food allergies and colitis. Recent studies highlighted the role of probiotics in regulating IEB functions and behavior in adults, but their effects in the newborn remain largely unknown. We therefore characterized in rat pups, the impact of Lactobacillus fermentum CECT 5716 (L. fermentum) on stress-induced IEB dysfunction, systemic immune response and exploratory behavior. Methods Newborn rats received daily by gavage either L. fermentum or water. Intestinal permeability to fluorescein sulfonic acid (FSA) and horseradish peroxidase (HRP) was measured following maternal separation (MS) and water avoidance stress (WAS). Immunohistochemical, transcriptomic, and Western blot analysis of zonula occludens-1 (ZO-1) distribution and expression were performed. Anxiety-like and exploratory behavior was assessed using the elevated plus maze test. Cytokine secretion of activated splenocytes was also evaluated. Key Results L. fermentum prevented MS and WAS-induced IEB dysfunction in vivo. L. fermentum reduced permeability to both FSA and HRP in the small intestine but not in the colon. L. fermentum increased expression of ZO-1 and prevented WAS-induced ZO-1 disorganization in ileal epithelial cells. L. fermentum also significantly reduced stress-induced increase in plasma corticosteronemia. In activated splenocytes, L. fermentum enhanced IFNγ secretion while it prevented IL-4 secretion. Finally, L. fermentum increased exploratory behavior. Conclusions & Inferences These results suggest that L. fermentum could provide a novel tool for the prevention and/or treatment of gastrointestinal disorders associated with altered IEB functions in the newborn.
Databáze: OpenAIRE
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