Correlation between granulocyte/macrophage-colony-forming units and CD34+ cells in apheresis products from patients treated with different chemotherapy regimens and granulocyte-colony-stimulating factor to mobilize peripheral blood progenitor cells
| Autor: | K. Höffken, K. Blumenstengel, C. Kunert, W. Vogel, H.-J. Fricke, R. Kath, Herbert G. Sayer |
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| Rok vydání: | 1998 |
| Předmět: |
Melphalan
Male Cancer Research Vincristine medicine.medical_treatment Prednisolone Antigens CD34 Cell Separation Pharmacology Dexamethasone Neoplasms Antineoplastic Combined Chemotherapy Protocols Granulocyte Colony-Stimulating Factor medicine Humans Ifosfamide Cyclophosphamide Etoposide Epirubicin Chemotherapy business.industry Macrophages Cytarabine General Medicine Flow Cytometry Hematopoietic Stem Cells Carmustine Hematopoietic Stem Cell Mobilization Granulocyte colony-stimulating factor Oncology Doxorubicin Immunology Blood Component Removal Female business medicine.drug Granulocytes |
| Zdroj: | Journal of cancer research and clinical oncology. 124(6) |
| ISSN: | 0171-5216 |
| Popis: | We examined the efficiency of disease-specific "standard" chemotherapies epirubicin, cyclophosphamide (EC); cyclophosphamide, vincristine, doxorubicin, etoposide, prednisolone (CHOEP); epirubicin, ifosfamide (EPI/IFOS) for peripheral blood progenitor cell (PBPC) mobilization in comparison to well-characterized mobilization protocols, i.e. etoposide, ifosfamide, cisplatin, epirubicin (VIPE) and dexamethasone, carmustine, etoposide, cytarabine, melphalan (DexaBEAM). Twenty-seven patients with various malignancies underwent 75 apheresis procedures for PBPC collection. Median cell yields from all 75 aphereses were 1.18 x 10(5) mononuclear cells/kg [range (0.28-3.7) x 10)8)], 1.4 x 10(5) granulocyte/macrophage-colony-forming units (CFU-GM)/kg [range (0.2-11) x 10(5)] and 3.3 x 10(6) CD34+cells/kg [range (0.35-17.7) x 10(6). CD34+/ CD90+ cells could be mobilized by all mobilization regimens used. The difference observed in the mobilization of CD34+ cells was only of low significance when the mobilization regimens were compared, whereas the mobilizations of MNC and CFU-GM were significantly different between the groups. Breast cancer patients treated with the VIPE regimen (including pretreated women) had a significantly higher CFU-GM rate than patients treated with EC (P=0.0005). Mobilized CD34+ PBPC were correlated with CFU-GM in all apheresis products. The linear correlation coefficients differed for the various mobilization groups: DexaBEAM (r=0.9, P0.0001), VIPE (r=0.68, P=0.0024), CHOEP (r=0.52, P=0.022), EPI/ IFOS (r=0.34, P=0.11) and EC (r=0.23, P=0.2). We conclude that clonogenic assays can provide additional information about the autotransplant quality, particularly when alternative or new mobilization regimens are being investigated. |
| Databáze: | OpenAIRE |
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