Potential role for IL-2 ELISpot in differentiating recent and remote infection in tuberculosis contact tracing
| Autor: | Norbert Reiling, A. Strassburg, Martin Ernst, B. Eker, Christoph Lange, Volker Schoellhorn, Benjamin Krummel, Heidrun Rath, Waltraud Wappler, Giovanni Sotgiu, Robert Hoerster, Andrea Glaewe |
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| Rok vydání: | 2009 |
| Předmět: |
Interleukin 2
Adult Male Infectious Diseases/Epidemiology and Control of Infectious Diseases Tuberculosis Adolescent Tuberculosis Contact lcsh:Medicine Tuberculin chemical and pharmacologic phenomena complex mixtures Cell Line Mycobacterium tuberculosis Infectious Diseases/Bacterial Infections Interferon-gamma Young Adult Tuberculosis diagnosis Medicine Humans Interferon gamma lcsh:Science Child Skin Tests Immunoassay Multidisciplinary biology business.industry ELISPOT Infectious Diseases/Respiratory Infections lcsh:R hemic and immune systems biology.organism_classification medicine.disease bacterial infections and mycoses Virology Logistic Models Immunology Interleukin-2 MED/01 Statistica medica lcsh:Q Female business medicine.drug Research Article |
| Zdroj: | PLoS ONE PLoS ONE, Vol 5, Iss 7, p e11670 (2010) |
| ISSN: | 1932-6203 |
| Popis: | Interferon (IFN)-gamma release assays (IGRA) have improved tuberculosis contact tracing, but discrimination of recent from remote Mycobacterium tuberculosis contacts is not possible by IGRA alone. We present results of a tuberculosis contact investigation with a new early-secretory-antigenic-target (ESAT)-6 and culture-filtrate-protein (CFP)-10 specific interleukin (IL)-2 ELISpot in addition to ESAT-6 and CFP-10 specific IFN-gamma ELISpot and tuberculin skin testing (TST). Results of the TST, IFN-gamma ELISpot and IL-2 ELISpot were positive in 6/172 (3.4%), 7/167 (4.2%) and 6/196 (3.1%) of contacts, respectively. Close contact (> or =100 hours) to the index case increased the risk of positive results in the IFN-gamma ELISpot, TST, and IL-2 ELISpot by 40.8, 19.3, and 2.5 times, respectively. Individuals with a positive IFN-gamma ELISpot/negative IL-2 ELISpot result had a median (IQR) duration of index case exposure of 568 hours (133_1000) compared to individuals with a positive IFN-gamma ELISpot/positive IL-2 ELISpot result (median = 24 hours; 20_130; p-value = 0.047). Combination of a M. tuberculosis specific IFN-gamma ELISpot with a M. tuberculosis specific IL-2 ELISpot significantly improved the identification of individuals with the highest risk of recent M. tuberculosis infection and is a promising method that should be explored to target tuberculosis preventive chemotherapy. |
| Databáze: | OpenAIRE |
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