Villin-Like Actin-Binding Proteins Are Expressed Ubiquitously in Arabidopsis
Autor: | Ulrich Klahre, Evelyne Friederich, Nam-Hai Chua, Daniel Louvard, Benedikt Kost |
---|---|
Rok vydání: | 2000 |
Předmět: |
Physiology
Molecular Sequence Data Arabidopsis macromolecular substances Plant Science digestive system DNA-binding protein Chlorocebus aethiops Tobacco Gene expression Genetics Animals Arabidopsis thaliana Amino Acid Sequence Actin-binding protein Vero Cells Actin Plant Proteins Microscopy Confocal Sequence Homology Amino Acid biology Binding protein Microfilament Proteins biology.organism_classification Plants Toxic Biochemistry biology.protein Carrier Proteins Villin Research Article |
Zdroj: | Plant Physiology. 122:35-48 |
ISSN: | 1532-2548 0032-0889 |
Popis: | In an attempt to elucidate the biological function of villin-like actin-binding proteins in plants we have cloned several genes encoding Arabidopsis proteins with high homology to animal villin. We found that Arabidopsis contains at least four villin-like genes (AtVLNs) encoding four different VLN isoforms. Two AtVLN isoforms are more closely related to mammalian villin in their primary structure and are also antigenically related, whereas the other two contain significant changes in the C-terminal headpiece domain. RNA and promoter/β-glucuronidase expression studies demonstrated that AtVLN genes are expressed in all organs, with elevated expression levels in certain types of cells. These results suggest that AtVLNs have less-specialized functions than mammalian villin, which is found only in the microvilli of brush border cells. Immunoblot experiments using a monoclonal antibody against pig villin showed that AtVLNs are widely distributed in a variety of plant tissues. Green fluorescent protein fused to full-length AtVLN and individual AtVLN headpiece domains can bind to both animal and plant actin filaments in vivo. |
Databáze: | OpenAIRE |
Externí odkaz: |