Short term storage of samples containing monoamines: ascorbic acid and glutathione give better protection against degradation than perchloric acid
| Autor: | Terje Sagvolden, Grete Wøien, Fernando Boix |
|---|---|
| Rok vydání: | 1997 |
| Předmět: |
Perchlorates
3 4-Dihydroxyphenylacetic acid Chromatography Antioxidant 5-Hydroxyindoleacetic acid Dopamine Drug Storage General Neuroscience medicine.medical_treatment Ascorbic Acid Glutathione Hydroxyindoleacetic Acid Ascorbic acid Biogenic Monoamines chemistry.chemical_compound chemistry medicine 3 4-Dihydroxyphenylacetic Acid Organic chemistry Degradation (geology) Perchloric acid Chromatography High Pressure Liquid medicine.drug |
| Zdroj: | Journal of Neuroscience Methods. 75:69-73 |
| ISSN: | 0165-0270 |
| DOI: | 10.1016/s0165-0270(97)00052-6 |
| Popis: | In order to study the protection of monoamines from degradation during short-time storage, the effect of three different antioxidants on the degradation of dopamine, dihydroxyphenylacetic acid (DOPAC), and 5-hydroxyindoleacetic acid (5-HIAA) was analyzed after 5 and 20 h. The results showed that dopamine was still quite stable after 20 h storage at room temperature, but that about 95% of 5-HIAA had disappeared. The best protection against degradation of all three substances was achieved when 15% v/v of a solution containing 1-2 mM ascorbic acid or 40 mM glutathione was added to the sample, resulting in near 100% protection after 20 h. Perchloric acid actually accelerated the degradation of 5-HIAA. |
| Databáze: | OpenAIRE |
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