3D Multilayered paper- and thread/paper-based microfluidic devices for bioassays
| Autor: | Ariana Gonzalez, Frank A. Gomez, Ricardo Guevara, Natalia M. Neris |
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| Rok vydání: | 2018 |
| Předmět: |
Paper
Cyan Clinical Biochemistry Microfluidics Iodide chemistry.chemical_element 02 engineering and technology Iodine 01 natural sciences Biochemistry Horseradish peroxidase Models Biological Analytical Chemistry Lab-On-A-Chip Devices Glucose oxidase Bradford protein assay chemistry.chemical_classification Wax Chromatography biology 010401 analytical chemistry Serum Albumin Bovine Equipment Design Microfluidic Analytical Techniques 021001 nanoscience & nanotechnology 0104 chemical sciences Glucose chemistry visual_art biology.protein visual_art.visual_art_medium Colorimetry 0210 nano-technology |
| Zdroj: | Electrophoresis. 40(2) |
| ISSN: | 1522-2683 |
| Popis: | In this paper we describe the fabrication of novel 3D microfluidic paper-based analytical devices (3D-μPADs) and a 3D microfluidic thread/paper-based analytical device (3D-μTPAD) to detect glucose and BSA through colorimetric assays. The 3D-μPAD and 3D-μTPAD consisted of three (wax, heat pressed wax-printed paper, single-sided tape) and four (hole-punched single-sided tape, blank chromatography circles, heat-pressed wax-printed paper, hole-punched single-sided tape containing trifurcated thread) layers, respectively. The saturation curves for each assay were generated for all platforms. For the glucose assay, a solution of glucose oxidase (GOx), horseradish peroxidase, and potassium iodide was flowed through each platform and, upon contact with glucose, generated a yellow-brown color indicative of the oxidation of iodide to iodine. For the protein assay, BSA was flowed through each device and, upon contact with citrate buffer and tetrabromophenol blue, resulted in a color change from yellow to blue. The devices were dried, scanned, and analyzed yielding a correlation between either yellow intensity and glucose concentration or cyan intensity and BSA concentration. A similar glucose assay, using unknown concentrations of glucose in artificial urine, was conducted and, when compared to the saturation curve, showed good correlation between the theoretical and actual concentrations (percent differences |
| Databáze: | OpenAIRE |
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