Evaluation of the Activity of the Essential Oil from an Ornamental Flower against Aedes aegypti: Electrophysiology, Molecular Dynamics and Behavioral Assays

Autor: Paulo Milet-Pinheiro, Rayane Cristine Santos da Silva, Kamilla A. Dutra, Geanne K.N. Santos, Jorge Iulek, Daniela Maria do Amaral Ferraz Navarro, Patrícia Cristina Bezerra-Silva
Jazyk: angličtina
Rok vydání: 2016
Předmět:
0301 basic medicine
Viral Diseases
Physiology
Epidemiology
Protein Conformation
Oviposition
lcsh:Medicine
Dengue virus
Disease Vectors
medicine.disease_cause
Molecular Dynamics
Biochemistry
Mosquitoes
law.invention
Dengue Fever
Dengue
chemistry.chemical_compound
0302 clinical medicine
Computational Chemistry
law
Reproductive Physiology
Aedes
Medicine and Health Sciences
Bioassay
Food science
Animal Anatomy
lcsh:Science
Multidisciplinary
biology
Behavior
Animal
Hematology
Dodecanal
Undecanal
Lipids
Body Fluids
Insects
Chemistry
Blood
Infectious Diseases
Physical Sciences
Insect Proteins
Female
Anatomy
Research Article
Neglected Tropical Diseases
Arthropod Antennae
Arthropoda
030231 tropical medicine
Aedes aegypti
Flowers
Molecular Dynamics Simulation
Odorant Binding Proteins
03 medical and health sciences
Zingiberaceae
Botany
medicine
Oils
Volatile
Animals
Animal Physiology
Humans
Essential oil
lcsh:R
fungi
Organisms
Etlingera elatior
Biology and Life Sciences
Proteins
biology.organism_classification
Tropical Diseases
Invertebrates
Insect Vectors
Electrophysiological Phenomena
030104 developmental biology
chemistry
lcsh:Q
Antennae (Animal Physiology)
Oils
Zoology
Zdroj: PLoS ONE
PLoS ONE, Vol 11, Iss 2, p e0150008 (2016)
ISSN: 1932-6203
Popis: Dengue fever has spread worldwide and affects millions of people every year in tropical and subtropical regions of Africa, Asia, Europe and America. Since there is no effective vaccine against the dengue virus, prevention of disease transmission depends entirely on regulating the vector (Aedes aegypti) or interrupting human-vector contact. The aim of this study was to assess the oviposition deterrent activity of essential oils of three cultivars of torch ginger (Etlingera elatior, Zingiberaceae) against the dengue mosquito. Analysis of the oils by gas chromatography (GC)-mass spectrometry revealed the presence of 43 constituents, of which α-pinene, dodecanal and n-dodecanol were the major components in all cultivars. Solutions containing 100 ppm of the oils exhibited oviposition deterrent activities against gravid Ae. aegypti females. GC analysis with electroantennographic detection indicated that the oil constituents n-decanol, 2-undecanone, undecanal, dodecanal, trans-caryophyllene, (E)-β-farnesene, α-humulene, n-dodecanol, isodaucene and dodecanoic acid were able to trigger antennal depolarization in Ae. aegypti females. Bioassays confirmed that solutions containing 50 ppm of n-dodecanol or dodecanal exhibited oviposition deterrent activities, while a solution containing the alcohol and aldehyde in admixture at concentrations representative of the oil presented an activity similar to that of the 100 ppm oil solution. Docking and molecular dynamics simulations verified that the interaction energies of the long-chain oil components and Ae. aegypti odorant binding protein 1 were quite favorable, indicating that the protein is a possible oviposition deterrent receptor in the antenna of Ae. aegypti.
Databáze: OpenAIRE
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