Placental Aromatase Is Deficient in Placental Ischemia and Preeclampsia
| Autor: | Carlos E. Irarrazabal, Pia Venegas-Araneda, Alejandra M. Guzmán-Rojas, Pedro P. España-Perrot, Sebastian E. Illanes, María González, Manuel Varas-Godoy, Aneta Dobierzewska, Alejandra Pérez-Sepúlveda, Lara J. Monteiro, H. Figueroa-Diesel, Macarena Palominos-Rivera |
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| Rok vydání: | 2015 |
| Předmět: |
Adult
medicine.medical_specialty Estrone Placenta lcsh:Medicine Gestational Age Preeclampsia chemistry.chemical_compound Aromatase Pre-Eclampsia Ischemia Pregnancy Cell Line Tumor Internal medicine medicine Animals Humans Testosterone Choriocarcinoma Prospective Studies RNA Messenger lcsh:Science Fetus Multidisciplinary Estradiol biology lcsh:R Androstenedione Estrogens medicine.disease Oxygen tension Endocrinology medicine.anatomical_structure chemistry Case-Control Studies embryonic structures Androgens biology.protein Premature Birth Gestation Female lcsh:Q Rabbits Research Article |
| Zdroj: | PLoS ONE, Vol 10, Iss 10, p e0139682 (2015) PLoS ONE PloSone journal Artículos CONICYT CONICYT Chile instacron:CONICYT |
| ISSN: | 1932-6203 |
| DOI: | 10.1371/journal.pone.0139682 |
| Popis: | Introduction Preeclampsia is a maternal hypertensive disorder with uncertain etiology and a leading cause of maternal and fetal mortality worldwide, causing nearly 40% of premature births delivered before 35 weeks of gestation. The first stage of preeclampsia is characterized by reduction of utero-placental blood flow which is reflected in high blood pressure and proteinuria during the second half of pregnancy. In human placenta androgens derived from the maternal and fetal adrenal glands are converted into estrogens by the enzymatic action of placental aromatase. This implies that alterations in placental steroidogenesis and, subsequently, in the functionality or bioavailability of placental aromatase may be mechanistically involved in the pathophysiology of PE. Methods Serum samples were collected at 32–36 weeks of gestation and placenta biopsies were collected at time of delivery from PE patients (n = 16) and pregnant controls (n = 32). The effect of oxygen tension on placental cells was assessed by incubation JEG–3 cells under 1% and 8% O2 for different time periods, Timed-mated, pregnant New Zealand white rabbits (n = 6) were used to establish an in vivo model of placental ischemia (achieved by ligature of uteroplacental vessels). Aromatase content and estrogens and androgens concentrations were measured. Results The protein and mRNA content of placental aromatase significantly diminished in placentae obtained from preeclamptic patients compared to controls. Similarly, the circulating concentrations of 17-β-estradiol/testosterone and estrone/androstenedione were reduced in preeclamptic patients vs. controls. These data are consistent with a concomitant decrease in aromatase activity. Aromatase content was reduced in response to low oxygen tension in the choriocarcinoma JEG–3 cell line and in rabbit placentae in response to partial ligation of uterine spiral arteries, suggesting that reduced placental aromatase activity in preeclamptic patients may be associated with chronic placental ischemia and hypoxia later in gestation. Conclusions Placental aromatase expression and functionality are diminished in pregnancies complicated by preeclampsia in comparison with healthy pregnant controls. |
| Databáze: | OpenAIRE |
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