Induction of arginine vasopressin-enhanced green fluorescent protein expression in the locus coeruleus following kainic acid-induced seizures in rats
| Autor: | Hiroaki Fujihara, Toru Ishikura, Toyoaki Ohbuchi, Akinori Hamamura, Miwako Todoroki, Kenji Hachisuka, Masaru Iwanaga, Akiko Katoh, Yoichi Ueta, Motoko Ohno |
|---|---|
| Rok vydání: | 2011 |
| Předmět: |
Male
endocrine system medicine.medical_specialty Vasopressin Kainic acid Tyrosine 3-Monooxygenase Arginine Physiology medicine.drug_class Recombinant Fusion Proteins Green Fluorescent Proteins Biology Green fluorescent protein Animals Genetically Modified Behavioral Neuroscience chemistry.chemical_compound Seizures Internal medicine medicine Animals Rats Wistar Kainic Acid Endocrine and Autonomic Systems Receptor antagonist Rats Up-Regulation Arginine Vasopressin Psychiatry and Mental health Neuropsychology and Physiological Psychology Endocrinology nervous system chemistry Locus coeruleus NMDA receptor Immunohistochemistry Female Locus Coeruleus Dizocilpine Maleate hormones hormone substitutes and hormone antagonists |
| Zdroj: | Stress. 15:435-442 |
| ISSN: | 1607-8888 1025-3890 |
| DOI: | 10.3109/10253890.2011.637185 |
| Popis: | Seizure causes autonomic, neuroendocrine and stress responses. We examined the effects of kainic acid (KA)-induced seizures on the expression of the arginine vasopressin (AVP)-enhanced green fluorescent protein (eGFP) in the locus coeruleus (LC), an area known to contain noradrenergic cells, in AVP-eGFP transgenic male and female rats, with the rationale to identify stressors which induce AVP synthesis in the LC. Subcutaneous (s.c.) administration of KA caused a progressive development of seizure behavior within 24 h. AVP-eGFP fluorescence in the LC was detected 6, 24, and 48 h and 1 week after administration of KA (12 mg/kg). From a nearly undetectable level, it reached a maximum at 48 h after s.c. administration of KA and returned to the basal levels after 2 weeks. AVP-eGFP fluorescence in the LC after s.c. administration of KA was significantly reduced by the pretreatment with MK-801 (nonselective N-methyl-D-aspartate (NMDA) receptor antagonist). In the KA-administered rats, immunohistochemistry for tyrosine hydroxylase (TH) revealed that the eGFP fluorescence was co-localized with TH-immuno-reactivity in the LC. These results suggest that the synthesis of AVP-eGFP is potentially up-regulated in noradrenergic neurons in the LC after KA-induced seizures through the activation of NMDA receptors. |
| Databáze: | OpenAIRE |
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