Peel of traditional apple varieties as a great source of bioactive compounds: extraction by micro-matrix solid-phase dispersion

Autor: Tihomir Kovač, Bojan Šarkanj, Ante Lončarić, Martina Skendrović Babojelić, Marta Lores, Katarina Matanović, Perla Ferrer
Přispěvatelé: Universidade de Santiago de Compostela. Departamento de Química Analítica, Nutrición e Bromatoloxía
Jazyk: angličtina
Rok vydání: 2020
Předmět:
Zdroj: Foods, Vol 9, Iss 1, p 80 (2020)
Minerva. Repositorio Institucional de la Universidad de Santiago de Compostela
instname
Foods
Volume 9
Issue 1
Popis: Micro matrix solid phase dispersion (micro-MSPD) was optimized by response surface methodology for the extraction of polyphenols from the peel of twelve traditional and eight commercial apple varieties grown in Croatia. The optimized micro-MSPD procedure includes the use of 0.2 g of sample, 0.8 g of dispersant, a 57% solution of methanol in water as the solvent and 5 mL of extract volume. The total polyphenolic index (TPI) and antioxidant activity (AA) were measured by spectrophotometric assays. Eighteen polyphenolic compounds were identified in all investigated apples by HPLC-DAD and LC-(ESI)-MS. The peel of traditional apple varieties had higher contents of all investigated polyphenols. Calculated relative contribution of polyphenol groups indicated non-flavonoids (28.6%) and flavanols (46.2%) as the major contributors to the total polyphenolic content in traditional and commercial apple varieties, respectively. The most abundant polyphenol in traditional apple peel was chlorogenic acid, procyanidin B2 and epicatechin (1143 ±
755 µ
g/g dw, 954 ±
343 µ
g/g dw and 560 ±
362 µ
g/g dw, respectively). The peel of varieties &lsquo
Apistar&rsquo
&lsquo
Bobovac&rsquo
and &lsquo
Božićnica&rsquo
could be highlighted as an important source of polyphenols.
Databáze: OpenAIRE