Comparison of bioaugmentation and biostimulation for the enhancement of dense nonaqueous phase liquid source zone bioremediation
Autor: | C. H. Ward, R. C. Daprato, Joanna Hughes, Diego E. Gomez, M. L. B. Da Silva, Pedro J. J. Alvarez |
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Rok vydání: | 2007 |
Předmět: |
DNA
Bacterial Bioaugmentation Tetrachloroethylene Electron donor Aquifer Biostimulation chemistry.chemical_compound Bioremediation Reductive dechlorination Environmental Chemistry Soil Pollutants Waste Management and Disposal Dissolution Water Science and Technology geography geography.geographical_feature_category Bacteria Reverse Transcriptase Polymerase Chain Reaction Ecological Modeling Environmental engineering Human decontamination Pollution Biodegradation Environmental chemistry Environmental chemistry Water Pollutants Chemical |
Zdroj: | Water environment research : a research publication of the Water Environment Federation. 78(13) |
ISSN: | 1061-4303 |
Popis: | Two 11.7-m 3 experimental controlled release systems (ECRS), packed with sandy model aquifer material and amended with tetrachloroethene (PCE) dense nonaqueous phase liquid (DNAPL) source zone, were operated in parallel with identical flow regimes and electron donor amendments. Hydrogen Releasing Compound (Regenesis Bioremediation Products, Inc., San Clemente, California), and later dissolved lactate, served as electron donors to promote dechlorination. One ECRS was bioaugmented with an anaerobic dechlorinating consortium directly into the source zone, and the other served as a control (biostimulated only) to determine the benefits of bioaugmentation. The presence of halorespiring bacteria in the aquifer matrix before bioaugmentation, shown by nested polymerase chain reaction with phylogenetic primers, suggests that dechlorinating catabolic potential may be somewhat widespread. Results obtained corroborate that source zone reductive dechlorination of PCE is possible at near field scale and that a system bioaugmented with a competent halorespiring consortium can enhance DNAPL dissolution and dechlorination processes at significantly greater rates than in a system that is biostimulated only. |
Databáze: | OpenAIRE |
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