Unveiling the heterogeneity of NKT cells in the liver through single cell RNA sequencing
Autor: | Xiaolong Liu, Hao Shen, Tao Liang, Haifeng Liu, Chan Gu, Fan Guo |
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Jazyk: | angličtina |
Rok vydání: | 2020 |
Předmět: |
0301 basic medicine
Liver cytology Cell Immunology lcsh:Medicine chemical and pharmacologic phenomena Biology Article Transcriptome 03 medical and health sciences 0302 clinical medicine Antigen T-Lymphocyte Subsets medicine Animals Cluster Analysis Secretion Lymphocytes lcsh:Science Cells Cultured Mice Knockout Multidisciplinary Sequence Analysis RNA Gene Expression Profiling lcsh:R Wild type hemic and immune systems Acquired immune system Natural killer T cell Cell biology Mice Inbred C57BL 030104 developmental biology medicine.anatomical_structure Liver Natural Killer T-Cells lcsh:Q Antigens CD1d Single-Cell Analysis 030215 immunology |
Zdroj: | Scientific Reports, Vol 10, Iss 1, Pp 1-13 (2020) Scientific Reports |
ISSN: | 2045-2322 |
Popis: | CD1d-dependent type I NKT cells, which are activated by lipid antigen, are known to play important roles in innate and adaptive immunity, as are a portion of type II NKT cells. However, the heterogeneity of NKT cells, especially NKT-like cells, remains largely unknown. Here, we report the profiling of NKT (NK1.1+CD3e+) cells in livers from wild type (WT), Jα18-deficient and CD1d-deficient mice by single-cell RNA sequencing. Unbiased transcriptional clustering revealed distinct cell subsets. The transcriptomic profiles identified the well-known CD1d-dependent NKT cells and defined two CD1d-independent NKT cell subsets. In addition, validation of marker genes revealed the differential organ distribution and landscape of NKT cell subsets during liver tumor progression. More importantly, we found that CD1d-independent Sca-1−CD62L+ NKT cells showed a strong ability to secrete IFN-γ after costimulation with IL-2, IL-12 and IL-18 in vitro. Collectively, our findings provide a comprehensive characterization of NKT cell heterogeneity and unveil a previously undefined functional NKT cell subset. |
Databáze: | OpenAIRE |
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