Direct effects of fermented cow's milk product with Lactobacillus paracasei CBA L74 on human enterocytes
| Autor: | Rita Nocerino, Cristina Bruno, C. Fierro, Lorella Paparo, Rosita Aitoro, R. Berni Canani |
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| Přispěvatelé: | Paparo, L, Nocerino, R, Fierro, C, Bruno, C, BERNI CANANI, Roberto |
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Microbiology (medical) beta-Defensins Lactobacillus paracasei fermented food Cultured Milk Products medicine.medical_treatment infectious disease Mucin 2 Occludin Microbiology Cathelicidin Tight Junctions 03 medical and health sciences 0302 clinical medicine Cathelicidins medicine Humans Receptor innate immunity Cell Proliferation Mucin-2 Innate immune system biology Cell growth intestinal permeability Probiotics Toll-Like Receptors Lactase Cell Differentiation Lacticaseibacillus paracasei biology.organism_classification 030104 developmental biology Enterocytes Gene Expression Regulation Zonula Occludens-1 Protein 030211 gastroenterology & hepatology Caco-2 Cells gut mucosa Antimicrobial Cationic Peptides |
| Popis: | Cow’s milk fermented with Lactobacillus paracasei CBA L74 (FM-CBAL74) exerts a preventive effect against infectious diseases in children. We evaluated if this effect is at least in part related to a direct modulation of non-immune and immune defence mechanisms in human enterocytes. Human enterocytes (Caco-2) were stimulated for 48 h with FM-CBAL74 at different concentrations. Cell growth was assessed by colorimetric assay; cell differentiation (assessed by lactase expression), tight junction proteins (zonula occludens1 and occludin), mucin 2, and toll-like receptor (TRL) pathways were analysed by real-time PCR; innate immunity peptide synthesis, beta-defensin-2 (HBD-2) and cathelicidin (LL-37) were evaluated by ELISA. Mucus layer thickness was analysed by histochemistry. FMCBA L74 stimulated cell growth and differentiation, tight junction proteins and mucin 2 expression, and mucus layer thickness in a dose-dependent fashion. A significant stimulation of HBD-2 and LL-37 synthesis, associated with a modulation of TLR pathway, was also observed. FM-CBAL74 regulates non-immune and immune defence mechanisms through a direct interaction with the enterocytes. These effects could be involved in the preventive action against infectious diseases demonstrated by this fermented product in children. |
| Databáze: | OpenAIRE |
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