Role of HLA class II and cytokine expression in rheumatoid arthritis
| Autor: | Marc Feldmann, Martin R Turner, A M Kissonerghis, G Buchan, C Haworth, A Ziegler, Ravinder N. Maini, K Barrett, Fionula M. Brennan, David Chantry |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
medicine.drug_class
medicine.medical_treatment T-Lymphocytes Immunology Antigen presentation Antigen-Presenting Cells Monoclonal antibody Arthritis Rheumatoid Biological Factors Rheumatology medicine Immunology and Allergy Humans RNA Messenger Antigen-presenting cell Messenger RNA business.industry Histocompatibility Antigens Class II General Medicine medicine.disease Blot Cytokine Rheumatoid arthritis Antibody Formation Cytokines Joints business Alpha chain |
| Zdroj: | Scopus-Elsevier |
| Popis: | HLA class II expression is notable in rheumatoid arthritis. We have investigated the mechanism of HLA class II regulation in the joints and found local synthesis, as judged by mRNA levels to be high. The role of antigen presentation in maintaining class II mRNA was explored, and blocking presentation by using monoclonal antibodies to HLA class II inhibited synthesis of mRNA for HLA-DR alpha chain. HLA class II expression is maintained by cytokines and so cytokine production in rheumatoid joints was investigated. It was chosen to use mRNA analysis by slot blotting as a screening assay, and the expression of many cytokines was detected. Levels of these were maintained in culture in the absence of extrinsic stimulation. |
| Databáze: | OpenAIRE |
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