A Roadmap to Cardiac Tissue‐Engineered Construct Preservation: Insights from Cells, Tissues, and Organs
Autor: | Linda W. van Laake, Jasmijn Janssen, Vasco Sampaio-Pinto, Paula M. Alves, Ilja K. Voets, Margarida Serra, Nino Chirico, Alain van Mil, Pieter A. Doevendans, Joost P.G. Sluijter |
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Jazyk: | angličtina |
Předmět: |
Materials science
antifreeze proteins heart failure hypothermic and normothermic preservation 02 engineering and technology cryopreservation 010402 general chemistry Bioinformatics 01 natural sciences Cryopreservation cardiac tissue engineering High doses Animals General Materials Science Preservation methods Tissue engineered Tissue Engineering Myocardium Mechanical Engineering cryoprotective agents Heart 021001 nanoscience & nanotechnology vitrification 0104 chemical sciences 3. Good health Transplantation Mechanics of Materials 0210 nano-technology Construct (philosophy) |
Zdroj: | Adv Mater |
ISSN: | 1521-4095 0935-9648 |
DOI: | 10.1002/adma.202008517 |
Popis: | Worldwide, over 26 million patients suffer from heart failure (HF). One strategy aspiring to prevent or even to reverse HF is based on the transplantation of cardiac tissue-engineered (cTE) constructs. These patient-specific constructs aim to closely resemble the native myocardium and, upon implantation on the diseased tissue, support and restore cardiac function, thereby preventing the development of HF. However, cTE constructs off-the-shelf availability in the clinical arena critically depends on the development of efficient preservation methodologies. Short- and long-term preservation of cTE constructs would enable transportation and direct availability. Herein, currently available methods, from normothermic- to hypothermic- to cryopreservation, for the preservation of cardiomyocytes, whole-heart, and regenerative materials are reviewed. A theoretical foundation and recommendations for future research on developing cTE construct specific preservation methods are provided. Current research suggests that vitrification can be a promising procedure to ensure long-term cryopreservation of cTE constructs, despite the need of high doses of cytotoxic cryoprotective agents. Instead, short-term cTE construct preservation can be achieved at normothermic or hypothermic temperatures by administration of protective additives. With further tuning of these promising methods, it is anticipated that cTE construct therapy can be brought one step closer to the patient. |
Databáze: | OpenAIRE |
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