de novo Blood Biomarkers in Autism: Autoantibodies against Neuronal and Glial Proteins
| Autor: | Abou-Donia, Mohamed B., Suliman, Hagir B., Siniscalco, Dario, Antonucci, Nicola, ElKafrawy, Passent, Brahmajothi, Mulugu V. |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
lcsh:BF1-990
neuronal and astroglial biomarkers autism spectrum disorder Development neuronal autoantibodies Article 03 medical and health sciences Behavioral Neuroscience 0302 clinical medicine Western blot mental disorders Genetics Medicine maternal autoantibodies General Psychology Ecology Evolution Behavior and Systematics 030304 developmental biology chemistry.chemical_classification 0303 health sciences Glial fibrillary acidic protein biology medicine.diagnostic_test business.industry Autoantibody medicine.disease control children Myelin basic protein lcsh:Psychology chemistry nervous system Immunology biology.protein Biomarker (medicine) Autism Antibody business Glycoprotein autoimmune disorder 030217 neurology & neurosurgery |
| Zdroj: | Behavioral Sciences Volume 9 Issue 5 Behavioral Sciences, Vol 9, Iss 5, p 47 (2019) |
| ISSN: | 2076-328X |
| Popis: | Autism spectrum disorders (ASDs) are the most common neurodevelopmental disorders with unidentified etiology. The behavioral manifestations of ASD may be a consequence of genetic and/or environmental pathology in neurodevelopmental processes. In this limited study, we assayed autoantibodies to a panel of vital neuronal and glial proteins in the sera of 40 subjects (10 children with ASD and their mothers along with 10 healthy controls, age-matched children and their mothers). Serum samples were screened using Western Blot analysis to measure immunoglobulin (IgG) reactivity against a panel of 9 neuronal proteins commonly associated with neuronal degeneration: neurofilament triplet proteins (NFP), tubulin, microtubule-associated proteins (tau), microtubule-associated protein-2 (MAP-2), myelin basic protein (MBP), myelin-associated glycoprotein (MAG), &alpha synuclein (SNCA) and astrocytes proteins such as glial fibrillary acidic protein (GFAP) and S100B protein. Our data show that the levels of circulating IgG class autoantibodies against the nine proteins were significantly elevated in ASD children. Mothers of ASD children exhibited increased levels of autoantibodies against all panel of tested proteins except for S100B and tubulin compared to age-matched healthy control children and their mothers. Control children and their mothers showed low and insignificant levels of autoantibodies to neuronal and glial proteins. These results strongly support the importance of anti-neuronal and glial protein autoantibodies biomarker in screening for ASD children and further confirm the importance of the involvement of the maternal immune system as an index that should be considered in fetal in utero environmental exposures. More studies are needed using larger cohort to verify these results and understand the importance of the presence of such autoantibodies in children with autism and their mothers, both as biomarkers and their role in the mechanism of action of autism and perhaps in its treatment. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
| Nepřihlášeným uživatelům se plný text nezobrazuje | K zobrazení výsledku je třeba se přihlásit. |