A method for characterizing adsorption of flowing solutes to microfluidic device surfaces
| Autor: | Kenneth R. Hawkins, Sandip Ghosal, Paul Yager, Richard R. Baldwin, Mark R. Steedman, Elain Fu |
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| Rok vydání: | 2007 |
| Předmět: |
Materials science
Time Factors Surface Properties Microfluidics Biomedical Engineering Analytical chemistry Bioengineering Biocompatible Materials Biochemistry chemistry.chemical_compound Adsorption Materials Testing Rhodamine B Deposition (phase transition) Molecule Bovine serum albumin Chromatography biology Rhodamines Serum Albumin Bovine General Chemistry Equipment Design Microfluidic Analytical Techniques Tetraethylene glycol dimethyl ether Surface coating Dextran chemistry Chemical engineering biology.protein Fluorescein-5-isothiocyanate |
| Zdroj: | Lab on a chip. 7(2) |
| ISSN: | 1473-0197 |
| Popis: | We present a method for characterizing the adsorption of solutes in microfluidic devices that is sensitive to both long-lived and transient adsorption and can be applied to a variety of realistic device materials, designs, fabrication methods, and operational parameters. We have characterized the adsorption of two highly adsorbing molecules (FITC-labeled bovine serum albumin (BSA) and rhodamine B) and compared these results to two low adsorbing species of similar molecular weights (FITC-labeled dextran and fluorescein). We have also validated our method by demonstrating that two well-known non-fouling strategies [deposition of the polyethylene oxide (PEO)-like surface coating created by radio-frequency glow discharge plasma deposition (RF-GDPD) of tetraethylene glycol dimethyl ether (tetraglyme, CH(3)O(CH(2)CH(2)O)(4)CH(3)), and blocking with unlabeled BSA] eliminate the characteristic BSA adsorption behavior observed otherwise. |
| Databáze: | OpenAIRE |
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