Lipids–Fluorophores Interactions Probed by Combined Nonlinear Polarized Microscopy
| Autor: | Paulina Gasecka, Naveen K. Balla, Miguel Sison, Sophie Brasselet |
|---|---|
| Přispěvatelé: | Institut FRESNEL (FRESNEL), Aix Marseille Université (AMU)-École Centrale de Marseille (ECM)-Centre National de la Recherche Scientifique (CNRS), Centre National de la Recherche Scientifique (CNRS)-École Centrale de Marseille (ECM)-Aix Marseille Université (AMU) |
| Rok vydání: | 2021 |
| Předmět: |
Microscopy
0303 health sciences Lipid Bilayers 02 engineering and technology 021001 nanoscience & nanotechnology 3. Good health Surfaces Coatings and Films Membrane Lipids 03 medical and health sciences Phosphatidylcholines [SPI.OPTI]Engineering Sciences [physics]/Optics / Photonic Materials Chemistry Physical and Theoretical Chemistry 0210 nano-technology ComputingMilieux_MISCELLANEOUS Fluorescent Dyes 030304 developmental biology |
| Zdroj: | Journal of Physical Chemistry B Journal of Physical Chemistry B, 2021, 125 (50), pp.13718-13729. ⟨10.1021/acs.jpcb.1c07866⟩ Journal of Physical Chemistry B, American Chemical Society, 2021, 125 (50), pp.13718-13729. ⟨10.1021/acs.jpcb.1c07866⟩ |
| ISSN: | 1520-5207 1520-6106 |
| Popis: | Studying the structural dynamics of lipid membranes requires methods that can address both microscopic and macroscopic characteristics. Fluorescence imaging is part of the most used techniques to study membrane properties in various systems from artificial membranes to cells: It benefits from a high sensitivity to local properties such as polarity and molecular orientational order, with a high spatial resolution down to the single-molecule level. The influence of embedded fluorescent lipid probes on the lipid membrane molecules is however poorly known and relies most often on molecular dynamics simulations, due to the challenges faced by experimental approaches to address the molecular-scale dimension of this question. In this work we develop an optical microscopy imaging method to probe the effect of fluorophores embedded in the membrane as lipid probes, on their lipid environment, with a lateral resolution of a few hundreds of nanometers. We combine polarized-nonlinear microscopy contrasts that can independently address the lipid probe, by polarized two-photon fluorescence, and the membrane lipids, by polarized coherent Raman scattering. Using trimethylamino derivative 1-(4-trimethylammonium-phenyl)-6-phenyl-1,3,5-hexatriene (TMA-DPH) and di-8-butyl-amino-naphthyl-ethylene-pyridinium-propyl-sulfonate (di-8-ANEPPS) as model probes, we show that both probes tend to induce an orientational disorder of their surrounding lipid CH-bonds in 1,2-dipalmitoylphosphatidylcholine (DPPC) lipids environments, while there is no noticeable effect in more disordered 1,2-dioleoyl |
| Databáze: | OpenAIRE |
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