Chemokines control fat accumulation and leptin secretion by cultured human adipocytes
| Autor: | Ignacio A. Romero, Arthur Donny Strosberg, Cindy C. Gerhardt, Raffaella Cancello, Luc Camoin |
|---|---|
| Rok vydání: | 2001 |
| Předmět: |
Leptin
Chemokine medicine.medical_specialty Leukocyte migration Adipose tissue Inflammation Biochemistry Chemokine receptor Endocrinology Internal medicine Adipocytes medicine Humans RNA Messenger Chemokine CCL4 Molecular Biology Cells Cultured Chemokine CCL2 Triglycerides Chemokine CCL3 biology Tumor Necrosis Factor-alpha Chemistry Monocyte Interleukin-8 Cell Differentiation Chemotaxis Macrophage Inflammatory Proteins Immunohistochemistry Up-Regulation medicine.anatomical_structure biology.protein Receptors Leptin Receptors Chemokine Chemokines medicine.symptom |
| Zdroj: | Molecular and Cellular Endocrinology. 175:81-92 |
| ISSN: | 0303-7207 |
| DOI: | 10.1016/s0303-7207(01)00394-x |
| Popis: | In addition to their role in inflammation, cytokines like TNFalpha have been reported to regulate the adipose tissue function suggesting a role for these soluble mediators in metabolism. However, it is not known whether adipocytes have the capacity to secrete chemokines, a group of low molecular weight inflammatory mediators that control leukocyte migration into tissues. Here we show that primary cultures of human preadipocytes constitutively produce three chemokines, interleukin-8 (IL-8), macrophage inflammatory protein-1alpha (MIP-1alpha) and monocyte chemotactic protein-1 (MCP-1), while their level of expression is low in mature adipocytes. Upon TNFalpha treatment, the expression of all the three chemokines is upregulated in adipocytes differentiated in vitro. In addition, we describe the presence of seven different chemokine receptors, mainly in mature adipocytes, both in vitro and in human fat tissue sections. Prolonged stimulation of cultured human adipocytes with exogenous chemokines leads to a decrease in lipid content in association with the downregulation of PPARgamma mRNA expression. Moreover, chemokines positively control the secretion of leptin, a hormone that regulates appetite, by a post-transcriptional mechanism. These findings reveal a new role for chemokines in the regulation of adipose tissue and suggest a novel therapeutic basis for the treatment of obesity, diabetes and cachexia. |
| Databáze: | OpenAIRE |
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