Cooperation of Endoderm-Derived BMP2 and Extraembryonic Ectoderm-Derived BMP4 in Primordial Germ Cell Generation in the Mouse
| Autor: | Ying Ying, Guang-Quan Zhao |
|---|---|
| Rok vydání: | 2001 |
| Předmět: |
Male
Heterozygote medicine.medical_specialty animal structures allantois Bmp2 Bone Morphogenetic Protein 2 Cell Count Bmp4 Ectoderm Bone Morphogenetic Protein 4 In situ hybridization Biology Bone morphogenetic protein 2 Mice Transforming Growth Factor beta Internal medicine medicine Animals endoderm Molecular Biology DNA Primers Mice Knockout mouse embryo Base Sequence Bmp8b Homozygote fungi Gene Expression Regulation Developmental Allantois Embryo Cell Biology biological factors Cell biology Mice Inbred C57BL Gastrulation Germ Cells medicine.anatomical_structure Endocrinology primordial germ cell Epiblast Bone Morphogenetic Proteins embryonic structures Female Endoderm Digestive System Abnormalities Developmental Biology |
| Zdroj: | Developmental Biology. 232:484-492 |
| ISSN: | 0012-1606 |
| DOI: | 10.1006/dbio.2001.0173 |
| Popis: | The primordial germ cells (PGCs) of the mouse are derived from proximal epiblast cells that are adjacent to the extraembryonic ectoderm during gastrulation. Previous studies have demonstrated that extraembryonic ectoderm-derived BMP4 and BMP8B are both required for PGC generation. Here we show that Bmp2, a member of the Dpp class of the Bmp superfamily, also plays a role in PGC generation. PGC number is significantly reduced in Bmp2 heterozygous and homozygous embryos at the N2 generation onto C57BL/6 background. Bmp2 homozygous embryos also have a short allantois and about 50% of them do not undergo normal chorioallantoic fusion. Using whole-mount in situ hybridization, we show that Bmp2 is primarily expressed in the endoderm of mouse pregastrula and gastrula embryos. Using a genetic approach, we further show that Bmp2 and Bmp4, but not Bmp2 and Bmp8b, have an additive effect on PGC generation. These results suggest that PGC generation in the mouse embryo is regulated not only by extraembryonic ectoderm-derived BMP4 and BMP8B, but also by endoderm-derived BMP2. |
| Databáze: | OpenAIRE |
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