Molecular properties and myocardial salvage effects of morin hydrate
| Autor: | Norman Camerman, Arthur A. Grey, Kwok-Pui Fung, Andrew Hempel, Jun Wu, Ling-Hua Zeng, Tai-Wing Wu |
|---|---|
| Rok vydání: | 1995 |
| Předmět: |
Xanthine Oxidase
Magnetic Resonance Spectroscopy Antioxidant Stereochemistry medicine.medical_treatment Chemical structure Coronary Disease Myocardial Reperfusion Morin Crystallography X-Ray Biochemistry Medicinal chemistry Antioxidants Mass Spectrometry Necrosis chemistry.chemical_compound medicine Animals Chelation Xanthine oxidase Flavonoids Pharmacology Chemistry Hydrogen bond Myocardium Heart Nuclear magnetic resonance spectroscopy Rabbits Trolox |
| Zdroj: | Biochemical Pharmacology. 49:537-543 |
| ISSN: | 0006-2952 |
| DOI: | 10.1016/0006-2952(94)00446-s |
| Popis: | Morin hydrate is a bioactive pigment found in yellow Brazil wood. Recently, we reported that morin hydrate prolongs the survival of three types of cells from the human circulatory system against oxyradicals generated in vitro. The protection excels that given by equimolar concentrations of ascorbate, mannitol, and Trolox. Here, we demonstrate that, in vivo, morin hydrate at 5 mumol/kg actually reduced by > 50% the tissue necrosis in post-ischemic and reperfused rabbit hearts. Mechanistically, morin hydrate not only scavenges oxyradicals, but also moderately inhibits xanthine oxidase, a free-radical generating enzyme from the ischemic endothelium. Among other possibilities, morin hydrate appears to chelate some metal ions (e.g. Fe2+) in oxyradical formation, although this needs to be examined further. Nuclear magnetic resonance (at 500 mHz) and electron-impact mass spectrometry also supported a molecular formula of C15H10O7 for morin hydrate. Only by X-ray crystallography was it clearly revealed that there are two water molecules attached by intermolecular hydrogen bonds to a morin molecule. Also, the three rings of morin hydrate approach coplanarity. This conformation favours a delocalization of electrons after oxyradical reduction, making morin an effective antioxidant. Thus, we have documented some of the molecular properties and myocardial salvage effects of morin hydrate. |
| Databáze: | OpenAIRE |
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