Harmonizing Cell-Free DNA Collection and Processing Practices through Evidence-Based Guidance
| Autor: | Mark D. Pertile, Sonya Parpart-Li, Sarah R. Greytak, Muhammed Murtaza, Helen M. Moore, Kelly B. Engel, Abel Jacobus Bronkhorst |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Cancer Research Evidence-based practice Quantification methods Computer science Best practice Guidelines as Topic Harmonization Biospecimen Collection Article Specimen Handling 03 medical and health sciences 0302 clinical medicine Basic research Neoplasms Biomarkers Tumor Animals Humans Research Liquid Biopsy Blood draw 030104 developmental biology Oncology Cell-free fetal DNA Risk analysis (engineering) Evidence-Based Practice 030220 oncology & carcinogenesis Cell-Free Nucleic Acids |
| Zdroj: | Clin Cancer Res |
| ISSN: | 1557-3265 1078-0432 |
| DOI: | 10.1158/1078-0432.ccr-19-3015 |
| Popis: | Circulating cell-free DNA (cfDNA) is rapidly transitioning from discovery research to an important tool in clinical decision making. However, the lack of harmonization of preanalytic practices across institutions may compromise the reproducibility of cfDNA-derived data and hamper advancements in cfDNA testing in the clinic. Differences in cellular genomic contamination, cfDNA yield, integrity, and fragment length have been attributed to different collection tube types and anticoagulants, processing delays and temperatures, tube agitation, centrifugation protocols and speeds, plasma storage duration and temperature, the number of freeze-thaw events, and cfDNA extraction and quantification methods, all of which can also ultimately impact subsequent downstream analysis. Thus, there is a pressing need for widely applicable standards tailored for cfDNA analysis that include all preanalytic steps from blood draw to analysis. The NCI's Biorepositories and Biospecimen Research Branch has developed cfDNA-specific guidelines that are based upon published evidence and have been vetted by a panel of internationally recognized experts in the field. The guidelines include optimal procedures as well as acceptable alternatives to facilitate the generation of evidence-based protocols by individual laboratories and institutions. The aim of the document, which is entitled “Biospecimen Evidence-based Best Practices for Cell-free DNA: Biospecimen Collection and Processing,” is to improve the accuracy of cfDNA analysis in both basic research and the clinic by improving and harmonizing practices across institutions. |
| Databáze: | OpenAIRE |
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