Modulation of Membrane Influx and Efflux in Escherichia coli Sequence Type 131 Has an Impact on Bacterial Motility, Biofilm Formation, and Virulence in a Caenorhabditis elegans Model
| Autor: | Alix Pantel, Jennifer Mesureur, Christelle Ngba Essebe, Jean-Marie Pagès, Jean-Philippe Lavigne, Albert Sotto, Catherine Dunyach-Remy, Marie-Hélène Nicolas-Chanoine |
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| Přispěvatelé: | Virulence bactérienne et maladies infectieuses (VBMI), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Montpellier (UM), Transporteurs membranaires, chimioresistance et drug-design (TMCD2), Aix Marseille Université (AMU)-Institut National de la Santé et de la Recherche Médicale (INSERM), Hôpital Beaujon, Assistance publique - Hôpitaux de Paris (AP-HP) (APHP)-Université Paris Diderot - Paris 7 (UPD7)-Hôpital Beaujon, Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Université Paris Diderot - Paris 7 (UPD7)-Hôpital Beaujon [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Hôpital Beaujon [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Université Paris Diderot - Paris 7 (UPD7) |
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Ertapenem Membrane permeability 030106 microbiology Mutant Motility Virulence Biology medicine.disease_cause beta-Lactams Microbiology 03 medical and health sciences Cefoxitin Mechanisms of Resistance medicine Escherichia coli Animals Pharmacology (medical) [SDV.BBM]Life Sciences [q-bio]/Biochemistry Molecular Biology Caenorhabditis elegans Pharmacology Biofilm Biological Transport [SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology Anti-Bacterial Agents Infectious Diseases Chloramphenicol Biofilms Mutation Efflux Bacterial outer membrane |
| Zdroj: | Antimicrobial Agents and Chemotherapy Antimicrobial Agents and Chemotherapy, American Society for Microbiology, 2016, 60 (5), pp.2901-2911. ⟨10.1128/AAC.02872-15⟩ |
| ISSN: | 0066-4804 1098-6596 |
| DOI: | 10.1128/AAC.02872-15⟩ |
| Popis: | Energy-dependent efflux overexpression and altered outer membrane permeability (influx) can promote multidrug resistance (MDR). The present study clarifies the regulatory pathways that control membrane permeability in the pandemic clone Escherichia coli sequence type 131 (ST131) and evaluates the impact of efflux and influx modulations on biofilm formation, motility, and virulence in the Caenorhabditis elegans model. Mutants of two uropathogenic E. coli (UPEC) strains, MECB5 (ST131; H 30-Rx) and CFT073 (ST73), as well as a fecal strain, S250 (ST131; H 22), were in vitro selected using continuous subculture in subinhibitory concentrations of ertapenem (ETP), chloramphenicol (CMP), and cefoxitin (FOX). Mutations in genes known to control permeability were shown for the two UPEC strains: MECB5-FOX (deletion of 127 bp in marR ; deletion of 1 bp and insertion of an IS 1 element in acrR ) and CFT073-CMP (a 1-bp deletion causing a premature stop in marR ). We also demonstrated that efflux phenotypes in the mutants selected with CMP and FOX were related to the AcrAB-TolC pump, but also to other efflux systems. Alteration of membrane permeability, caused by underexpression of the two major porins, OmpF and OmpC, was shown in MECB5-ETP and mutants selected with FOX. Lastly, our findings suggest that efflux pump-overproducing isolates (CMP mutants) pose a serious threat in terms of virulence (significant reduction in worm median survival) and host colonization. Lack of porins (ETP and FOX mutants) led to a high level of antibiotic resistance in an H 30-Rx subclone. Nevertheless, this adaptation created a physiological disadvantage (decreased motility and ability to form biofilm) associated with a low potential for virulence. |
| Databáze: | OpenAIRE |
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