Evaluation of mass spectrometric techniques for characterization of engineered proteins
| Autor: | Karl H. Schram, Peter Roepstorff, Kate Rafn, Kjeld Poulsen, Trausti Baldursson, Karsten Kristiansen, Jens Knudsen, Jenny Krøll, Jens S. Andersen |
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| Rok vydání: | 1995 |
| Předmět: |
Molecular Sequence Data
Mutagenesis (molecular biology technique) Bioengineering Sequence (biology) Peptide Mass spectrometry Protein Engineering Applied Microbiology and Biotechnology Biochemistry Peptide Mapping Mass Spectrometry law.invention law Amino Acid Sequence Molecular Biology Chromatography High Pressure Liquid chemistry.chemical_classification Diazepam Binding Inhibitor Nitrogen Isotopes Chemistry Binding protein Lasers Intact protein Mass spectrometric Recombinant Proteins Evaluation Studies as Topic Spectrometry Mass Matrix-Assisted Laser Desorption-Ionization Mutation Recombinant DNA Carrier Proteins Biotechnology |
| Zdroj: | Roepstorff, P, Schram, K H, Andersen, J S, Rafn, K, Baldursson, T, Krøll, J, Poulsen, K, Knudsen, J & Kristiansen, K 1995, ' Evaluation of mass spectrometric techniques for characterization of engineered proteins. ', Molecular Biotechnology, vol. 4, no. 1, pp. 1-12 . https://doi.org/10.1007/BF02907466 |
| ISSN: | 1073-6085 |
| DOI: | 10.1007/BF02907466 |
| Popis: | Udgivelsesdato: 1995-Aug Mass spectrometric characterization of engineered proteins has been examined using bovine recombinant Acyl-CoA-Binding Protein (rACBP), [15N]-labeled rACBP, and a number of sequence variants of ACBP produced by site-directed mutagenesis. The mass spectrometric techniques include ESIMS and MALDIMS for analysis of the intact protein. Peptide maps have been obtained either by direct analysis of enzymatically derived mixtures by PDMS, ESIMS, and MALDIMS or by off- and on-line HPLC-mass spectrometry. ESIMS was found to be most accurate for analysis of intact proteins. The best sequence coverage in mapping was obtained by LC-ESIMS and by direct mixture analysis by MALDIMS. The latter technique was favorable in terms of sensitivity and speed. A general strategy for mass spectrometric characterization of engineered proteins is suggested. |
| Databáze: | OpenAIRE |
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