Impact of LINE-1 hypomethylation on the clinicopathological and molecular features of colorectal cancer patients

Autor:	Pei Ching Lin, Jen Kou Lin, Hung Hsin Lin, Wei Shone Chen, Tai Chuan Kuan, Shung Haur Yang, Jeng Kai Jiang, Wen Yi Liang, Shih Ching Chang, Chun Chi Lin, Yuan Tzu Lan
Jazyk:	angličtina
Rok vydání:	2018
Předmět:	0301 basic medicine Oncology Male Multivariate analysis Colorectal cancer Physiology lcsh:Medicine Artificial Gene Amplification and Extension Biochemistry Polymerase Chain Reaction 0302 clinical medicine Mathematical and Statistical Techniques Medicine and Health Sciences lcsh:Science Aged 80 and over Univariate analysis Multidisciplinary DNA methylation Chemical Reactions Methylation Middle Aged Chromatin Body Fluids Nucleic acids Survival Rate Chemistry Blood 030220 oncology & carcinogenesis Physical Sciences Epigenetics Female Microsatellite Instability Anatomy DNA modification Colorectal Neoplasms Chromatin modification Statistics (Mathematics) Research Article Chromosome biology medicine.medical_specialty Cell biology Histology Colon Research and Analysis Methods Disease-Free Survival 03 medical and health sciences Internal medicine Statistical significance medicine Genetics Humans Statistical Methods Molecular Biology Techniques Survival rate Molecular Biology Aged Neoplasm Staging Colorectal Cancer Biology and life sciences business.industry lcsh:R Microsatellite instability Cancers and Neoplasms DNA medicine.disease Gastrointestinal Tract 030104 developmental biology Long Interspersed Nucleotide Elements Multivariate Analysis lcsh:Q Gene expression business Digestive System Mathematics Microsatellite Repeats
Zdroj:	PLoS ONE, Vol 13, Iss 5, p e0197681 (2018) PLoS ONE
ISSN:	1932-6203
Popis:	Recent studies suggest that aberrant DNA methylation might occur early and commonly in colorectal tumorigenesis. In 111 normal subjects, the mean LINE-1 methylation level of peripheral blood was 81.0 ± 5.7%. Of 143 colorectal cancer (CRC) patients, the mean level of LINE-1 methylation was 60.5 ± 12.5%. We defined below 60% as cut-off value of LINE-1 hypomethylation, and 93 cases (65.0%) had LINE-1 hypomethylation in the tumor tissue. LINE-1 hypomethylation was not associated with any other clinical features. There was a trend that LINE-1 hypomethylation tumors were associated with advanced disease, but it did not reach statistical significance. There was no significant association between mutations of 12 genes, MSI-high, EMAST, and LINE-1 hypomethylation level. The median follow-up was 61.2 months. Five-year disease-free survival (DFS) and overall survival curves of patients with LINE-1 hypomethylation tumors were significantly lower than those of patients with normal LINE-1 methylation tumors (p = 0.032 and 0.001, respectively). Multivariate analysis showed that only TNM staging was an independent prognostic factor for CRC patients including DFS and overall survival (OS). LINE-1 did not impact patients' outcomes in multivariate analysis including DFS and OS. In conclusion, LINE-1 hypomethylation is marginally related to advanced stage CRC and impacts patients' outcomes in univariate analysis.
Databáze:	OpenAIRE
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