Immunofluorescence testing in the diagnosis of autoimmune blistering diseases: overview of 10-year experience
Autor: | Denise Miyamoto, Valeria Aoki, Tarsila Gasparotto Nogueira, Samia Trigo Arbache, Livia Delgado |
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Jazyk: | angličtina |
Rok vydání: | 2014 |
Předmět: |
Epidermolysis bullosa acquisita
Male Pathology medicine.medical_specialty Linear IgA bullous dermatosis Autoimmune diseases Fluorescent Antibody Technique Dermatology Biology Immunofluorescence Sensitivity and Specificity Skin Diseases medicine Humans IgA pemphigus skin and connective tissue diseases Pemphigoid benign mucous membrane Pemphigoid gestationis Retrospective Studies Skin Tests Investigation medicine.diagnostic_test integumentary system Reproducibility of Results IIf medicine.disease Immunoglobulin A Pemphigus Paraneoplastic pemphigus Skin diseases vesiculobullous Immunoglobulin G RL1-803 Female Epidermolysis bullosa Pemphigoid bullous |
Zdroj: | Anais Brasileiros de Dermatologia, Vol 89, Iss 6, Pp 885-889 (2014) Anais Brasileiros de Dermatologia, Volume: 89, Issue: 6, Pages: 885-889, Published: DEC 2014 Anais Brasileiros de Dermatologia v.89 n.6 2014 Anais brasileiros de dermatologia Sociedade Brasileira de Dermatologia (SBD) instacron:SBD Anais Brasileiros de Dermatologia |
ISSN: | 0365-0596 |
Popis: | BACKGROUND: Immunofluorescence testing is an important tool for diagnosing blistering diseases. OBJECTIVE: To characterize the immunofluorescence findings in patients diagnosed with autoimmune blistering skin diseases. METHODS: We retrospectively analyzed immunofluorescence results encompassing a 10-year period. RESULTS: 421 patients were included and divided into 2 groups: group 1- intraepidermal blistering diseases (n=277) and 2- subepidermal blistering diseases (n=144). For group 1, positive DIF findings demonstrated: predominance of IgG intercellular staining (ICS) and C3 for pemphigus foliaceus-PF (94% and 73% respectively), pemphigus vulgaris-PV (91.5%-79.5%) and paraneoplastic pemphigus-PNP (66%-33%); ICS IgA in 100% of IgA pemphigus cases, and IgG deposits in the basement membrane zone (BMZ) along with ICS in one Hailey-Hailey patient. The IIF findings revealed mean titers of 1:2.560 for PV and 1:1.280 for PF. For paraneoplastic pemphigus, IIF was positive in 2 out of 3 cases with rat bladder substrate. In group 2, positive DIF findings included multiple deposits at basement membrane zone for epidermolysis bullosa acquisita-EBA (C3-89%,IgG-79%,IgA-47%,IgM-21%) mucous membrane pemphigoid-MMP (C3,IgG,IgA,IgM-80%) and bullous pemphigoid-BP (C3-91%,IgG-39%,IgA-11%,IgM-6%), and IgA at basement membrane zone for IgA linear disease (99%) and dermatitis herpetiformis-DH (dermal papillae in 84.6%). For lichen planus pemphigoides, there was C3 (100%) and IgG (50%) deposition at basement membrane zone. indirect immunofluorescence positive findings revealed basement membrane zone IgG deposits in 46% of BP patients, 50% for EBA, 15% for IgA linear dermatosis and 50% for LPP. Indirect immunofluorescence positive results were higher for BP and EBA with Salt-Split skin substrate. CONCLUSION: Our results confirmed the importance of immunofluorescence assays in diagnosing autoimmune blistering diseases, and higher sensitivity for indirect immunofluorescence when Salt-split skin technique is performed. |
Databáze: | OpenAIRE |
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