Characterization of a Calcitonin Gene Related Peptide-Like Molecule in the Abalone, Haliotis tuberculata
| Autor: | E Lopez, M. Fouchereau-Peron, L. Duvail |
|---|---|
| Rok vydání: | 1997 |
| Předmět: |
food.ingredient
Abalone Calcitonin Gene-Related Peptide Immunology Radioimmunoassay In Vitro Techniques Calcitonin gene-related peptide Biology Iodine Radioisotopes Radioligand Assay food Animals Humans Molecule Haliotis Mantle (mollusc) Pharmacology integumentary system Proteins Molecular biology Rats Membrane Liver nervous system Mollusca Rat liver Chromatography Gel |
| Zdroj: | Comparative Biochemistry and Physiology Part C: Pharmacology, Toxicology and Endocrinology. 116:155-159 |
| ISSN: | 0742-8413 |
| Popis: | Immunoreactive related CGRP molecules (ir-CGRP) were identified in the abalone, Haliotis tuberculata, mainly in mantle and cephalic part extracts. Ir-CGRP in both tissues accounted for 461 and 455.6 pg per mg of proteins, respectively. These CGRP-immunoreactive molecules were further analyzed for their ability to interact with the CGRP radioreceptor assay. In specific target tissues for CGRP (rat liver membranes), 50% inhibition of 125I-labeled CGRP specific binding was observed with 4.7 micrograms and 21.1 micrograms of proteins from mantle and cephalic part extract, respectively. These molecules were submitted to gel-filtration chromatography on a Sephacryl S-100 column and were further analyzed in the radioreceptor assay specific for CGRP. The elution position of these molecules suggested a molecular weight close to that of synthetic salmon calcitonin. |
| Databáze: | OpenAIRE |
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