Effect of PA-MSHA vaccine on plasma phospholipids metabolic profiling and the ratio of Th2/Th1 cells within immune organ of mouse IgA nephropathy
| Autor: | Lewen Jia, Jun Yang, Guowang Xu, Hongwei Kong, Fanglou Li, Shen Lv, Chang Wang |
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| Rok vydání: | 2006 |
| Předmět: |
medicine.medical_specialty
Spectrometry Mass Electrospray Ionization Clinical Biochemistry Pharmaceutical Science Analytical Chemistry Nephropathy Enterotoxins Mice Random Allocation Immune system Th2 Cells Pharmacokinetics Immunity Tandem Mass Spectrometry Immunopathology Internal medicine Drug Discovery Blood plasma medicine Animals Least-Squares Analysis Spectroscopy Chromatography High Pressure Liquid Phospholipids Mice Inbred BALB C Principal Component Analysis Chemistry Reproducibility of Results Glomerulonephritis Glomerulonephritis IGA Serum Albumin Bovine Th1 Cells medicine.disease Lipid Metabolism Bacterial vaccine Disease Models Animal Endocrinology Bacterial Vaccines Pseudomonas aeruginosa Female Spleen |
| Zdroj: | Journal of pharmaceutical and biomedical analysis. 43(2) |
| ISSN: | 0731-7085 |
| Popis: | Phospholipids as a class of important constituents in the biomembranes have been paid increasing attention in many fields. IgA nephropathy is now generally known to be the most common form of primary glomerulonephritis in the world. However, phospholipids metabolism in IgA nephropathy was not clear. Until recently, there was no effective treatment available for patients with IgA nephropathy. In this paper, effect of PA-MSHA vaccine on plasma phospholipid metabolic profile of mouse IgA nephropathy was investigated using high performance liquid chromatography/mass spectrometry (HPLC/MS) and principal components analysis (PCA). Female Balb/c mice were divided into four groups: model group, control group, PA-MSHA treatment group and medicine control group (dipyridamole+common threewingnut root). The experimental IgA nephropathy model was established by the immunity combination method of oral BSA and injection of SEB. It was found that combination of LC/MS technology with PCA can be successfully applied to phospholipids profile analysis, clearly classify the model group and normal group, and PA-MSHA treatment group is closer to the normal control group than medicine control group. The result showed that Th(2)/Th(1) (=CD(4)(+)CD(30)(+)/CD(4)(+)CD(30)(-)) of the model group is 20.70+/-3.57, which is significantly higher than that of the control group (1.34+/-0.14) (P |
| Databáze: | OpenAIRE |
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