Delayed wound healing in Mac-1?deficient mice is associated with normal monocyte recruitment

Autor: Jon E. Mogford, Mark Sisco, Injoong Kim, Tanya N. Mayadas, Jerome D. Chao, Thomas A. Mustoe
Rok vydání: 2007
Předmět:
Zdroj: Wound Repair and Regeneration. 15:566-571
ISSN: 1524-475X
1067-1927
DOI: 10.1111/j.1524-475x.2007.00264.x
Popis: The Mac-1 integrin is an important mediator of migration and inflammatory activation of neutrophils and monocytes. However, the role of Mac-1 in modulating macrophage emigration and activation and its subsequent impact on cutaneous wound healing have not been fully elucidated. To examine the significance of Mac-1 to murine wound healing, we measured epithelialization and granulation tissue formation in partial-thickness ear wounds and full-thickness head wounds, respectively, in Mac-1-deficient mice. Wounds were histologically analyzed at postwounding days 3, 5, and 7. The gap measured between the leading edges of inward-migrating granulation tissue was significantly increased in knockout mice compared with control animals at day 5 (3.8+/-0.3 vs. 2.6+/-0.5 mm; p0.001) and day 7 (2.2+/-0.4 vs. 0.96+/-0.73 mm; p=0.005). Epithelial gap measurements were also increased in knockout mice vs. wild-type controls at days 3 (0.62+/-0.02 vs. 0.54+/-0.07 mm; p0.05) and 5 (0.58+/-0.06 vs. 0.39+/-0.08 mm; p0.001). Immunohistochemistry showed equal numbers of macrophages in knockout and control wounds. These findings show that Mac-1 is required for normal wound healing but that the attenuation in the deposition of granulation tissue and wound epithelialization in Mac-1 knockout mice is not associated with decreased monocyte migration into the wound.
Databáze: OpenAIRE
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