Specific heterologous F(ab')2 antibodies revert blood incoagulability resulting from envenoming by Lonomia obliqua caterpillars
| Autor: | W. D. Dias Da Silva, Luis Roberto de Camargo Gonçalves, J. E. Oliveira, Hisako Gondo Higashi, I. Fernandes, A. C. M. Rocha-Campos, I. K. Yamagushi, M. T. C. P. Ribela, Maria Cristina Cirillo Sousa-e-Silva |
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| Rok vydání: | 2001 |
| Předmět: |
Antivenom
Lonomia obliqua Heterologous Biology Moths Median lethal dose Lethal Dose 50 Immunoglobulin Fab Fragments Mice Antigen Neutralization Tests Virology Potency Animals Tissue Distribution Mice Inbred BALB C Venoms Blood Coagulation Disorders biology.organism_classification Infectious Diseases Immunology Antibody Formation biology.protein Parasitology Electrophoresis Polyacrylamide Gel Antibody Lonomia |
| Zdroj: | The American journal of tropical medicine and hygiene. 64(5-6) |
| ISSN: | 0002-9637 |
| Popis: | Contact with Lonomia obliqua caterpillars results in a bleeding syndrome characterized by hemorrhage and blood coagulation disturbances. Conventional therapy using antifibrinolytics or cryoprecipitates has been unable to treat pathophysiologic alterations. As antivenoms are effective therapy for treatment of victims of venomous animals, a process of manufacturing a specific antilonomic serum by immunizing horses with Lonomia caterpillar bristle extracts (LBE) was developed. Lonomia caterpillar bristle extracts exhibited several protein bands on SDS-PAGE, induced blood coagulation abnormalities and lethality in mice, and stimulated specific antibody production in horses. Sera obtained from immunized horses were rich in anti-LBE specific antibodies distributed among the horse IgG isotypes. These antibodies had the ability to recognize various LBE antigens as well as to neutralize their coagulopathy-inducing activity. The antivenom manufactured by the developed process was composed of purified and sterilized F(ab')2 with ED50 = 38.61 microl, potency = 0.29 mg/ml, and 95% confidence limit of potency 0.20-1.36. |
| Databáze: | OpenAIRE |
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