Regulation of cardiac mitochondrial calcium by average extramitochondrial calcium
| Autor: | D. A. Scott, J. R. Leisey, Russell C. Scaduto, L. W. Grotyohann |
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| Rok vydání: | 1993 |
| Předmět: |
Fura-2
Physiology Fluorescence spectrometry chemistry.chemical_element Mitochondrion Calcium Biology Mitochondria Heart chemistry.chemical_compound Physiology (medical) Animals Fluorescent Dyes Culture Media Extracellular Matrix Perfusion Cytosol Microscopy Fluorescence chemistry Biochemistry Mitochondrial matrix Biophysics Steady state (chemistry) NAD+ kinase Cardiology and Cardiovascular Medicine NADP |
| Zdroj: | American Journal of Physiology-Heart and Circulatory Physiology. 265:H1203-H1208 |
| ISSN: | 1522-1539 0363-6135 |
| DOI: | 10.1152/ajpheart.1993.265.4.h1203 |
| Popis: | A system to perifuse isolated rat heart mitochondria was designed to study the relationship between mitochondrial matrix free Ca2+ and extramitochondrial free Ca2+ under conditions in which the latter concentration could oscillate over a range typical of that expected in vivo. We tested the hypothesis that the level of intramitochondrial Ca2+ responds to the average extramitochondrial Ca2+ in the heart. Mitochondria were immobilized within an optical chamber for measurement of endogenous NAD(P)H and fura 2 fluorescence. NAD(P)H increased significantly on provision of substrates and decreased reversibly in the presence of ADP, indicating maintenance intact coupled respiration by this preparation. Matrix free Ca2+ was measured using fura 2-loaded mitochondria and, in parallel experiments, media free Ca2+ was measured with fura 2 in the absence of mitochondria. Oscillation of extramitochondrial Ca2+ from < 0.1 microM to approximately 2 microM at frequencies of 0.5, 1.0, and 1.25 cycles/s produced steady-state levels of matrix Ca2+ that were independent of frequency but proportional to the average media free Ca2+ concentration. Matrix Ca2+ increased to a steady state on an increase in the extramitochondrial average Ca2+ concentration with a half-time (t1/2) of approximately 2 min at 22 degrees C. Oscillation of mitochondrial Ca2+ was not observed under any conditions tested. The data are taken to indicate that in vivo, the concentration of mitochondrial matrix free Ca2+ is a steady state that is proportional to the average extramitochondrial Ca2+ concentration and that changes in the latter represent a mechanism of signal transduction from the cytosol to the mitochondrial matrix. |
| Databáze: | OpenAIRE |
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