Fingerprinting of glycans as their 2-aminoacridone derivatives by capillary electrophoresis and laserinduced fluorescence
| Autor: | Gary B. Harland, George E. Chapman, George Okafo, Ian C. Sellick, Paul Matejtschuk, Patrick Camilleri |
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| Rok vydání: | 1996 |
| Předmět: |
Glycan
medicine.drug_class Molecular Sequence Data Clinical Biochemistry Monoclonal antibody Biochemistry Fluorescence Analytical Chemistry chemistry.chemical_compound Capillary electrophoresis Polysaccharides medicine Animals Humans Laser-induced fluorescence Chromatography biology Aminoacridines Chemistry Lasers Electrophoresis Capillary Fetuin Sialic acid carbohydrates (lipids) Carbohydrate Sequence Immunoglobulin G biology.protein Cattle alpha-Fetoproteins Antibody |
| Zdroj: | Electrophoresis. 17:406-411 |
| ISSN: | 1522-2683 0173-0835 |
| Popis: | Capillary electrophoresis and laser-induced fluorescence detection have been used to fingerprint the 2-aminoacridone derivatives of complex glycans released from bovine fetuin and human IgG monoclonal antibodies. The utility of this method in distinguishing between N- and O-linked oligosaccharides and in determining the presence of sialic acid residues in glycan mixtures at an early stage of analysis has been demonstrated. |
| Databáze: | OpenAIRE |
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