A novel variant of translation elongation factor-1β: isolation and characterization of the rice gene encoding EF-1β2
| Autor: | Mitsuo Yamaki, Yasuyuki Kuroiwa, Takeshi Sawazaki, Ken-ichi Tsutsumi, Shin-ichiro Ejiri, Shin-ichiro Kidou, Yoshiaki Terui |
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| Rok vydání: | 1998 |
| Předmět: |
Gene isoform
Protein subunit Molecular Sequence Data Biophysics Biology Genes Plant Biochemistry Exon Peptide Elongation Factor 1 Structural Biology Complementary DNA Genetics Animals Amino Acid Sequence Gene Genomic Library Messenger RNA Sequence Homology Amino Acid Molecular mass Genetic Variation food and beverages Oryza Translation (biology) Peptide Elongation Factors Molecular biology Molecular Weight Artemia Sequence Alignment |
| Zdroj: | Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression. 1442:369-372 |
| ISSN: | 0167-4781 |
| DOI: | 10.1016/s0167-4781(98)00153-5 |
| Popis: | A rice gene encoding a novel isoform of translation elongation factor-1beta subunit (termed EF-1beta2) was isolated and characterized. The gene comprises of eight exons, and encodes a 226-amino-acid protein. Expression of EF-1beta2 mRNA is abundant in seeds and cultured cells, but is considerably low in the tissues of the rice seedling. Antiserum raised against an EF-1beta2 synthetic peptide detected a protein with a relative molecular mass of about 32 kDa, indicating the EF-1beta2 gene is actually expressed in rice tissues. EF-1beta2 showed a close similarity to the cognate subunits from plant (beta and beta'). |
| Databáze: | OpenAIRE |
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