Seeing the light: Shifting from wild rhizomes to extraction of active ingredients from above-ground parts of Paris polyphylla var. yunnanensis
Autor: | Chang-Xiang Chen, Wei Ni, Hai-Yang Liu, Xu-Jie Qin |
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Rok vydání: | 2017 |
Předmět: |
Conservation of Natural Resources
Staphylococcus aureus Candida parapsilosis Platelet Aggregation Cell Survival Saponin 01 natural sciences Anti-Infective Agents Cell Line Tumor Drug Discovery Candida albicans Escherichia coli Liliaceae Animals Humans Pharmacology chemistry.chemical_classification Active ingredient Traditional medicine biology 010405 organic chemistry Plant Extracts Aspergillus fumigatus Extraction (chemistry) Paris polyphylla Plant Components Aerial Saponins biology.organism_classification Antimicrobial Cytostatic Agents Polyphylla 0104 chemical sciences Rhizome Plant Leaves 010404 medicinal & biomolecular chemistry chemistry Pseudomonas aeruginosa Rabbits Paris polyphylla var. yunnanensis |
Zdroj: | Journal of ethnopharmacology. 224 |
ISSN: | 1872-7573 |
Popis: | Ethnopharmacological relevance The dried rhizomes of Paris polyphylla var. yunnanensis are widely used in traditional Chinese medicine (TCM) as hemostatic, antitumor, and antimicrobial agents. More than 70 Chinese patent medicines are based on P. polyphylla var. yunnanensis rhizomes. Steroidal saponins are considered as the main active ingredients of these rhizomes. However, wild populations of P. polyphylla var. yunnanensis are greatly threatened due to the illegal wild harvest and over-utilization of the rhizomes. In contrast, the renewable above-ground parts (leaves and stems) of P. polyphylla var. yunnanensis are usually thrown away as waste material, whether from wild or cultivated material. Aim of the study The aim of this study was to use HPLC analyses of chemical constituents and bioactive assays to assess whether the above-ground parts could be an alternative source of active ingredients to the rhizomes of P. polyphylla var. yunnanensis. Materials and methods The saponin components of the rhizomes and above-ground parts of P. polyphylla var. yunnanensis were analyzed by HPLC-UV. The total saponins extracted from the rhizomes and above-ground parts of P. polyphylla var. yunnanensis were evaluated for their hemostatic, cytotoxic, and antimicrobial activities by using the rabbit blood in vitro based on turbidimetric method, MTT assay method, and a dilution antimicrobial susceptibility test method, respectively. Results Four bioactive spirostanol saponins (paris saponins I, II, VI, and VII) were detected in the total saponins from the rhizomes and above-ground parts of P. polyphylla var. yunnanensis, which indicated they should have similar pharmacological properties. The bioactive assays revealed that both the parts of P. polyphylla var. yunnanensis exhibited the same hemostatic, cytotoxic, and antimicrobial effects. Conclusion Our results revealed that based on saponin content in the above-ground parts of P. polyphylla var. yunnanensis and the requirements stipulated in 2015 of Chinese Pharmacopoeia, the above-ground parts (especially its leaves) can be an alternative and more sustainable source of active ingredients compared to the rhizomes. |
Databáze: | OpenAIRE |
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